| Topic | Key finding | Organism/strain | Evidence type | Quantitative data | Citation ID | Publication year | URL (if in text) |
|---|---|---|---|---|---|---|---|
| Verified identity | PP_3002 is explicitly labeled as an aroE-2 locus in a KT2440-derived transcriptomics study, supporting assignment of the target as the P. putida KT2440 aroE/shikimate dehydrogenase distinct from aroE-1/other homologs | *Pseudomonas putida* KT2440 / S12 comparative context | Genome annotation / transcriptomics context | Locus noted as PP3002 (aroE-2) | (pqac-00000008) | 2008 | https://doi.org/10.1128/JB.01379-07 |
| Enzyme function | AroE is the archetypal shikimate dehydrogenase catalyzing the reversible NADP-dependent interconversion of 3-dehydroshikimate and shikimate in the shikimate pathway for aromatic amino acid biosynthesis | *Pseudomonas putida* KT2440 (family-level functional assignment with KT2440 homolog context) | Biochemical/functional characterization summary | Cofactor preference: NADPH/NADP+; substrate: shikimate/3-dehydroshikimate | (pqac-00000004, pqac-00000003) | 2012 | — |
| Pathway role | The shikimate pathway supplies chorismate and downstream aromatic amino acids; engineering studies in *P. putida* treat SDH/AroE as part of the central route controlling flux to anthranilate and other aromatics | *Pseudomonas putida* KT2440 | Pathway mapping / metabolic engineering | Not directly quantified for AroE in this row | (pqac-00000007, pqac-00000006) | 2015, 2024 | https://doi.org/10.3389/fmicb.2015.01310; https://doi.org/10.1101/2024.07.06.602327 |
| aroE knockout phenotype | KT2440 aroE knockout grows in rich LB but with impaired growth and delayed lag; fails to grow on succinate minimal medium, indicating AroE is required under minimal conditions | *Pseudomonas putida* KT2440 | Gene knockout phenotype | Lag extended by ~4.5 h; example OD600 after 15 h WT 1.467 vs aroE KO 1.079; other examples WT 2.431 vs KO 1.608 and WT 2.514 vs KO 1.748 | (pqac-00000000, pqac-00000005) | 2012 | — |
| Rescue/supplementation tests | Growth defect of aroE knockout was not rescued by single aromatic amino acids or tested shikimate-pathway intermediates in initial experiments | *Pseudomonas putida* KT2440 | Nutritional rescue experiments | No rescue with Trp, Phe, Tyr or tested intermediates under stated conditions | (pqac-00000000, pqac-00000001) | 2012 | — |
| Additional phenotype | aroE knockout lost characteristic WT UV fluorescence on King B medium, implying broader physiological consequences of disrupting this shikimate-pathway step | *Pseudomonas putida* KT2440 | Phenotypic observation | Qualitative loss of 365 nm fluorescence | (pqac-00000000) | 2012 | — |
| 2023 applied context | KT2440 was engineered to reverse gallic acid metabolism and produce gallic acid from glycerol via a synthetic operon linked to shikimate-pathway precursors | *Pseudomonas putida* KT2440 | Metabolic engineering application | 346.7 ± 0.004 mg L−1 gallic acid after 72 h | (pqac-00000005) | 2023 | https://doi.org/10.1007/s10123-022-00282-5 |
| 2024 applied context | A new-to-nature shikimate pathway-dependent catabolism (SDC) in *P. putida* rerouted catabolism through chorismate-linked pyruvate release, highlighting the strategic importance of shikimate-pathway flux | *Pseudomonas putida* | Metabolic modeling + engineering preprint | CHRPL-as-sole-pyruvate-source predicted growth rate 17.8% below WT and 26.1% faster than other shikimate-derived pyruvate-releasing reactions; native metabolism 1 mol pyruvate and 3.75 mol ATP per mol glycerol vs SDC 0.55 mol pyruvate and 0.23 mol ATP | (pqac-00000006) | 2024 | https://doi.org/10.1101/2024.07.06.602327 |
| 2024 product application | In a *Pseudomonas* aromatic-production study, limited shikimate-pathway precursor availability and diversion of shikimate toward dead-end 3-hydroxyshikimate were identified as barriers during 2-phenylethanol production | *Pseudomonas putida* DOT-T1E-derived strains | Metabolic engineering / process study | 2-PE up to ~120 ppm in engineered overproducer; 82–110 mg L−1 from 2G hydrolysates; 10.5–84.1 mg L−1 from corn syrup-derived assays | (pqac-00000009) | 2024 | https://doi.org/10.1186/s13068-024-02498-1 |
| 2015 product application | KT2440 anthranilate production was improved by manipulating upstream shikimate-pathway control (feedback-insensitive AroG plus TrpES40FG), illustrating practical use of flux through the AroE-containing pathway | *Pseudomonas putida* KT2440 | Metabolic engineering / bioreactor study | 0.25 ± 0.004 g L−1 anthranilate in optimized shake flasks; up to 1.54 ± 0.3 g L−1 in tryptophan-limited fed-batch; alternative feed gave 1.0 ± 0.07 g L−1 | (pqac-00000007) | 2015 | https://doi.org/10.3389/fmicb.2015.01310 |


*Table: This table summarizes identity verification, core enzymatic function, KT2440-specific knockout phenotypes, and recent applied shikimate-pathway contexts relevant to aroE/PP_3002. It is useful as a compact evidence map separating direct gene-specific findings from broader pathway-engineering studies in Pseudomonas.*