bcsB

UniProt ID: Q88JL3
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

bcsB encodes a single-pass inner-membrane/periplasmic BcsB-family accessory subunit of the bacterial cellulose synthase complex. It associates with the BcsA catalytic subunit and helps organize the periplasmic path for nascent cellulose during c-di-GMP-controlled cellulose biosynthesis.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005886 plasma membrane
IEA
GO_REF:0000120 		ACCEPT
Summary: Plasma membrane is the appropriate bacterial inner-membrane location for BcsB.
Reason: UniProt places BcsB in the cell inner membrane as a single-pass membrane protein.
Supporting Evidence:
file:PSEPK/bcsB/bcsB-uniprot.txt
SUBCELLULAR LOCATION: Cell inner membrane
file:PSEPK/bcsB/bcsB-goa.tsv
GO:0005886 plasma membrane
GO:0006011 UDP-alpha-D-glucose metabolic process
IEA
GO_REF:0000002 		MODIFY
Summary: UDP-alpha-D-glucose metabolism is an over-broad donor-substrate process for BcsB.
Reason: BcsB is a cellulose synthase regulatory subunit in bacterial cellulose biosynthesis; the broad UDP-glucose metabolic-process term should be replaced by the pathway process.
Proposed replacements: cellulose biosynthetic process
Supporting Evidence:
file:PSEPK/bcsB/bcsB-uniprot.txt
PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
file:PSEPK/bcsB/bcsB-goa.tsv
GO:0006011 UDP-alpha-D-glucose metabolic process
GO:0016020 membrane
IEA
GO_REF:0000002 		MODIFY
Summary: Membrane is correct but less specific than the bacterial plasma membrane assignment already present.
Reason: BcsB is specifically assigned to the cell inner membrane, so GO:0005886 is the preferred location term.
Proposed replacements: plasma membrane
Supporting Evidence:
file:PSEPK/bcsB/bcsB-uniprot.txt
SUBCELLULAR LOCATION: Cell inner membrane
file:PSEPK/bcsB/bcsB-goa.tsv
GO:0016020 membrane

Core Functions

Inner-membrane/periplasmic BcsB accessory role in the cellulose synthase complex, supporting BcsA-dependent cellulose polymerization and trans-envelope passage of the nascent glucan.

Directly Involved In:
cellulose biosynthetic process
Cellular Locations:
plasma membrane
Supporting Evidence:
  • file:PSEPK/bcsB/bcsB-uniprot.txt
    SUBUNIT: Tightly associated with the cellulose synthase catalytic
  • file:PSEPK/bcsB/bcsB-uniprot.txt
    PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
  • PMID:23222542
    BcsB is a periplasmic protein that is anchored to the inner membrane via a single, C-terminal TM-helix.
  • file:PSEPK/bcsB/bcsB-deep-research-openscientist.md
    BcsB is a periplasmic scaffold/adapter, not an enzyme.

References

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Suggested Questions for Experts

Q: Does KT2440 BcsB directly bind c-di-GMP, or is its regulatory role indirect through BcsA and local c-di-GMP-metabolism partners?

Q: Which local bcs-locus accessory proteins are required for BcsB-dependent cellulose synthesis and export?

Suggested Experiments

Experiment: Reconstitute KT2440 BcsA/BcsB membrane complexes and test whether BcsB variants affect cellulose synthase activity or BcsA PilZ-dependent c-di-GMP activation.

Type: in vitro regulatory and complex-assembly assay

Experiment: Compare cellulose production and biofilm matrix phenotypes in wild-type, bcsB deletion, and bcsB complementation strains.

Type: targeted genetics and biofilm polysaccharide assay

Deep Research

OpenScientist

(bcsB-deep-research-openscientist.md)

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