cbcX

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0015871 choline transport	IEA GO_REF:0000002	ACCEPT	Summary: CbcX supplies choline specificity to the Cbc importer. Reason: Experimental work on orthologous Pseudomonas Cbc systems establishes CbcX-dependent choline uptake. Supporting Evidence: PMID:19919675 CbcXWV is sufficient to transport choline and betaine but not carnitine
GO:0022857 transmembrane transporter activity	IEA GO_REF:0000002	REMOVE	Summary: CbcX contributes substrate recognition but does not itself span the membrane. Reason: The permease CbcW forms the membrane path; the periplasmic CbcX binding protein contributes to transporter activity. Supporting Evidence: PMID:19919675 The core transporter CbcWV also interacts with the carnitine-specific SBP CaiX (K(m), 24 microM) and the betaine-specific SBP BetX (K(m), 0.6 microM).
GO:0015220 choline transmembrane transporter activity	ISS PMID:19919675 The ATP-binding cassette transporter Cbc (choline/betaine/ca...	NEW	Summary: CbcX contributes substrate recognition to choline transport by the Cbc complex. Reason: The binding protein is one component of the multi-subunit transporter and does not enable transport alone. Supporting Evidence: PMID:19919675 The core transporter CbcWV also interacts with the carnitine-specific SBP CaiX (K(m), 24 microM) and the betaine-specific SBP BetX (K(m), 0.6 microM).
GO:0033265 choline binding	IEA GO_REF:0000002	ACCEPT	Summary: Choline binding is the direct molecular function of CbcX. Reason: Orthologous CbcX proteins bind choline preferentially and deliver it to CbcWV. Supporting Evidence: PMID:19919675 The SBP encoded by the cbcXWV operon, CbcX, binds choline with a high affinity (K(m), 2.6 microM)
GO:0042597 periplasmic space	IEA GO_REF:0000002	ACCEPT	Summary: The signal-peptide-bearing substrate receptor acts in the periplasm. Reason: CbcX is a soluble substrate-binding component of a Gram-negative ABC importer. Supporting Evidence: file:PSEPK/cbcX/cbcX-uniprot.txt SIGNAL 1..21
GO:0043190 ATP-binding cassette (ABC) transporter complex	IEA GO_REF:0000002	ACCEPT	Summary: CbcX is the interchangeable choline-binding component of the Cbc ABC importer. Reason: It docks with the CbcWV core to support substrate uptake. Supporting Evidence: PMID:19919675 a core transporter preferentially interacting with ligand-bound versus ligand-free SBPs file:PSEPK/cbcX/cbcX-deep-research-openscientist.md CbcX is the periplasmic substrate-binding protein (SBP)
GO:0055085 transmembrane transport	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: This broad process is valid but less informative than choline transport. Reason: CbcX participates in transmembrane uptake through the Cbc complex, but choline transport is the useful process term. Supporting Evidence: PMID:19919675 The Cbc transporter of Pseudomonas spp. is required for the uptake of QACs