Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000166 nucleotide binding	IEA GO_REF:0000104	MARK AS OVER ANNOTATED	Summary: True but nonspecific relative to ATP binding and ATP-dependent helicase activity. Reason: DnaB is specifically an ATP-dependent P-loop helicase, so generic nucleotide binding adds little value.
GO:0003677 DNA binding	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct substrate interaction for a replicative DNA helicase. Reason: DnaB binds and translocates on DNA while unwinding the parental duplex.
GO:0003678 DNA helicase activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct core molecular function. Reason: The local UniProt record identifies DnaB as the main ATP-dependent replicative DNA helicase.
GO:0004386 helicase activity	IEA GO_REF:0000104	MARK AS OVER ANNOTATED	Summary: Correct but broader than the DNA-specific helicase terms. Reason: GO:0003678 and GO:0043139 capture the relevant substrate and directionality.
GO:0005524 ATP binding	IEA GO_REF:0000120	ACCEPT	Summary: Correct nucleotide-binding property required for helicase action. Reason: DnaB is an ATP-dependent P-loop helicase.
GO:0005829 cytosol	IEA GO_REF:0000118	ACCEPT	Summary: Appropriate localization for the bacterial replicative helicase. Reason: DnaB operates at chromosome-replication forks in the cytosol/nucleoid.
GO:0006260 DNA replication	IEA GO_REF:0000120	ACCEPT	Summary: Correct core biological process. Reason: DnaB unwinds parental DNA during both initiation and elongation of chromosome replication.
GO:0006269 DNA replication, synthesis of primer	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: The target record places DnaB in the primosome and assigns primer synthesis, but helicase activity remains its defining role. Reason: The biological-process annotation does not assert that DnaB catalyzes RNA synthesis. The target UniProt record assigns both primosome-complex membership and this primer-synthesis process, supporting retention as non-core without inferring an undocumented target-specific interaction. Supporting Evidence: file:PSEPK/dnaB/dnaB-uniprot.txt GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
GO:0016787 hydrolase activity	IEA GO_REF:0000104	MARK AS OVER ANNOTATED	Summary: True but uninformative parent activity. Reason: ATP hydrolysis and directional DNA helicase activity provide the relevant mechanistic specificity.
GO:0016887 ATP hydrolysis activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct ATPase activity coupled to DNA unwinding. Reason: The local UniProt record explicitly describes DNA-dependent ATP hydrolysis by DnaB.
GO:0043139 5'-3' DNA helicase activity	IEA GO_REF:0000003	ACCEPT	Summary: Correct specific core activity and translocation polarity. Reason: DnaB is a processive 5'-to-3' DNA helicase at the bacterial replication fork.

Core Functions

ATP-dependent 5'-to-3' replicative helicase that unwinds parental duplex DNA ahead of the chromosome-replication fork.

Molecular Function:

5'-3' DNA helicase activity

Directly Involved In:

DNA replication

Cellular Locations:

cytosol

References

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📚 Additional Documentation

Notes

(dnaB-notes.md)

dnaB curation notes

Annotation-reviewer pass (2026-09-01)

Consulted the annotation-reviewer workflow against all 11 current GOA rows, UniProt Q88DF2, EC 5.6.2.3, and the DnaB domain assignments. Accepted DNA helicase activity, ATP binding/hydrolysis, cytosolic localization, DNA replication, and the specific 5'-3' helicase activity; retained generic DNA binding as non-core. Marked generic nucleotide binding, helicase activity, and hydrolase activity as over-annotated. Primer synthesis is retained as non-core because the target record assigns that process and primosome-complex membership, without using those automated assignments to infer a target-specific interaction mechanism. No GOA row remains pending.

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