eda

Summary: This annotation captures the specific catalytic activity named in UniProt and matches the recorded EC 4.1.2.14 reaction that cleaves KDPG into glyceraldehyde 3-phosphate and pyruvate. It is the core molecular function of Eda.

Reason: Q88P29 matches the KDPG aldolase-specific NCBIfam model TIGR01182 (eda), the Pfam Aldolase domain and both KDPG/KHG aldolase PROSITE active-site signatures. That family membership supports the KDPG aldolase activity characterised biochemically and structurally for E. coli and T. maritima family members (PMID:16403639). The UniProt reaction line rests only on an ARBA rule and is not cited. The gene sits in the zwf-pgl-eda operon, cloned from P. putida strain H and highly similar to the KT2440 copy, whose third gene encodes KDPG aldolase (PMID:12393206). No KT2440-specific kinetic assay is cached, so this is family-level support.

Summary: This broad parent term is technically correct because Eda is a lyase, but it adds little beyond the much more informative child term GO:0008675. It is a generic InterPro2GO (GO_REF:0000002) parent of the specific KDPG aldolase activity that is already annotated, so it represents an over-annotation rather than an informative independent function. The falcon deep research confirms the specific KDPG aldolase activity as the evolved function, leaving the generic lyase term redundant.

Reason: The specific child term GO:0008675 already captures the evolved aldolase (lyase) activity, so the generic parent lyase term is a redundant high-level IEA over-annotation that should not be propagated as a distinct function.

Summary: The UniProt pathway statement places Eda in 2-dehydro-3-deoxy-D-gluconate degradation step 2/2, which is the terminal aldol-cleavage step of the Entner-Doudoroff pathway. This process-level annotation is missing from GOA and should be added. (Previously proposed as GO:0009255 Entner-Doudoroff pathway through 6-phosphogluconate, which GO obsoleted with replaced_by GO:0061678.)

Reason: Eda itself performs a step of the Entner-Doudoroff route, the KDPG aldolase cleavage that produces pyruvate and glyceraldehyde 3-phosphate from KDPG, so the process term reflects participation, not mere requirement. The activity rests on family membership (NCBIfam TIGR01182 eda) and on the mechanism established for E. coli and T. maritima KDPG aldolases (PMID:16403639). The eda gene is co-transcribed with zwf and pgl, upstream enzymes of the same route, in a carbohydrate-induced operon (PMID:12393206, strain H operon highly similar to KT2440). KT2440 glycolysis runs almost exclusively through the ED route (PMID:26350459), which gives organismal context but does not name eda. The UniProt PATHWAY line rests only on an ARBA rule, and the eda mutant phenotype reported in deep research is not in the cached abstract of PMID:23967821, so neither is cited.