EutC (PP_0542) is the small beta subunit of the adenosylcobalamin-dependent ethanolamine ammonia-lyase. It associates with the EutB large subunit to form the active enzyme, completes the intersubunit cobalamin-binding environment, and contributes to conversion of ethanolamine into acetaldehyde and ammonium.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0006520 amino acid metabolic process | IEA GO_REF:0000002 | REMOVE | Summary: Ethanolamine is an amino alcohol, and its catabolism is not an amino acid metabolic process. Reason: This InterPro mapping is a category over-transfer. GO:0046336 directly captures the established pathway and is already present. Supporting Evidence: PMID:19783625 Ethanolamine-ammonia lyase, the enzyme that breaks ethanolamine into acetaldehyde and ammonia, is encoded by the gene tandem eutBC. |
| GO:0008851 ethanolamine ammonia-lyase activity | IEA GO_REF:0000120 | ACCEPT | Summary: The ethanolamine ammonia-lyase term is correct for the EutB/EutC enzyme; EutC contributes to it as the non-catalytic small subunit. Reason: The reaction is correctly assigned to the EutB/EutC enzyme. E. coli structural evidence places the active-site barrel in EutB and the lower cobalamin-contact surface in EutC; conserved EutC membership supports a contribution-qualified core for KT2440. This does not establish that every source enables relation is categorically wrong: GO catalytic-subunit semantics do not require isolated-subunit activity. The combined electronic source receives a probable, source-specific qualifier caution, with its original annotation payload preserved. Supporting Evidence: PMID:20519496 The active site is in the (beta/alpha)(8) barrel of the alpha-subunit; the beta-subunit covers the lower part of the cobalamin that is bound in the interface of the alpha- and beta-subunits. PMID:19762342 The eutB and eutC genes in the eut operon encoded the large and small subunits of the enzyme, respectively. The enzyme existed as the heterododecamer alpha(6)beta(6). |
| GO:0009350 ethanolamine ammonia-lyase complex | IEA GO_REF:0000120 | ACCEPT | Summary: EutC is the small subunit of the EutB/EutC enzyme complex. Reason: EutB and EutC encode the large and small subunits, and purified E. coli enzyme forms an alpha6-beta6 complex. The exact GOA PTN is PTN002446609; canonical local PAINT data independently place GO:0009350 at PTN002217404 using P19636 and P19265 as seeds. Supporting Evidence: PMID:19762342 The eutB and eutC genes in the eut operon encoded the large and small subunits of the enzyme, respectively. The enzyme existed as the heterododecamer alpha(6)beta(6). file:PSEPK/eutC/eutC-uniprot.txt Pfam; PF05985; EutC; 1. file:PSEPK/eutC/eutC-deep-research-openscientist.md EutC is the small (Ξ²) subunit of ethanolamine ammonia-lyase |
| GO:0031419 cobalamin binding | IEA GO_REF:0000104 | ACCEPT | Summary: EutC makes direct contacts with the cobalamin cofactor bound at the EutB/EutC interface. Reason: In the characterized E. coli ortholog EutC carries experimentally defined adenosylcobalamin-contact residues (UniProt positions 207, 228 and 258, from the crystal structures) and covers the lower face of the cofactor. It binds cobalamin directly, so `enables` is acceptable, as for EutB. Supporting Evidence: PMID:20519496 the beta-subunit covers the lower part of the cobalamin that is bound in the interface of the alpha- and beta-subunits file:ECOLI/eutC/eutC-uniprot.txt Note=Binds 1 AdoCbl between the large and small subunits, with 6 cofactors per holoenzyme. |
| GO:0031469 bacterial microcompartment | IEA GO_REF:0000044 | REMOVE | Summary: The conventional Eut microcompartment location is not supported for the KT2440 reference proteome. Reason: The reproducible KT2440 reference-proteome inventory detects no PF00936/IPR000249 BMC or PF03319/IPR004992 vertex signatures, with the E. coli Eut shell set recovered as a positive control; the local eut locus also lacks identified shell genes. This supports rejecting a conventional Eut shell-location transfer to KT2440. The inference is bounded by signature coverage and could miss divergent shell proteins. It does not demonstrate where native KT2440 EutBC activity occurs or establish a free cytosolic active state. Supporting Evidence: file:PSEPK/eutC/eutC-bioinformatics/RESULTS.md KT2440 encodes no BMC-domain or EutN/CcmL-domain protein anywhere in its reference proteome, while the same queries recover the complete E. coli Eut shell set. |
| GO:0031471 ethanolamine degradation polyhedral organelle | IEA GO_REF:0000104 | REMOVE | Summary: The conventional Eut microcompartment location is not supported for the KT2440 reference proteome. Reason: The reproducible KT2440 reference-proteome inventory detects no PF00936/IPR000249 BMC or PF03319/IPR004992 vertex signatures, with the E. coli Eut shell set recovered as a positive control; the local eut locus also lacks identified shell genes. This supports rejecting a conventional Eut shell-location transfer to KT2440. The inference is bounded by signature coverage and could miss divergent shell proteins. It does not demonstrate where native KT2440 EutBC activity occurs or establish a free cytosolic active state. Supporting Evidence: file:PSEPK/eutC/eutC-bioinformatics/RESULTS.md KT2440 encodes no BMC-domain or EutN/CcmL-domain protein anywhere in its reference proteome, while the same queries recover the complete E. coli Eut shell set. |
| GO:0046336 ethanolamine catabolic process | IEA GO_REF:0000120 | ACCEPT | Summary: EutC is an obligate subunit of the pathway-defining ethanolamine ammonia-lyase. Reason: The EutBC tandem defines the conversion of ethanolamine to acetaldehyde and ammonium across diverse bacterial ethanolamine-utilization systems. Supporting Evidence: PMID:19783625 Ethanolamine-ammonia lyase, the enzyme that breaks ethanolamine into acetaldehyde and ammonia, is encoded by the gene tandem eutBC. |
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Download this section (compressed HTML)Q: Where does active EutBC occur in native KT2440, and how is acetaldehyde loss or toxicity controlled? Does the adjacent aldB-I aldehyde dehydrogenase couple to the enzyme despite the absence of detected conventional BMC shell signatures?
Experiment: Grow wild type, eutC deletion and complemented strains on ethanolamine with B12, measure ethanolamine ammonia-lyase activity and acetaldehyde release, and determine EutC localization by controlled fractionation or imaging rather than presupposing a soluble location.
Type: reverse genetics, enzyme assay and cell fractionation
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