flgM encodes the anti-sigma-28 factor that negatively regulates flagellin gene transcription by binding FliA until flagellar assembly has reached the appropriate stage.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0045892 negative regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: This annotation is appropriate. FlgM is the anti-sigma-28 factor that prevents FliA-dependent transcription of flagellin genes until flagellar assembly is ready. Reason: Negative regulation of DNA-templated transcription captures the direct anti-sigma regulatory effect. Supporting Evidence: file:PSEPK/flgM/flgM-uniprot.txt Responsible for the coupling of flagellin expression to file:PSEPK/flgM/flgM-uniprot.txt preventing expression of the flagellin genes file:PSEPK/flgM/flgM-goa.tsv GO:0045892 negative regulation of DNA-templated transcription file:PSEPK/flgM/flgM-deep-research-falcon.md FlgM is a **cytoplasmic anti-σ factor** that antagonizes **FliA (σ28)**, thereby acting as a **negative regulator of late flagellar gene expression** (including flagellin/filament genes). file:PSEPK/flgM/flgM-deep-research-falcon.md PP_4395** is explicitly annotated as **“Negative regulator of flagellin synthesis FlgM”** and is downregulated when FliA is absent, matching the canonical FliA–FlgM regulatory pair. PMID:27663176 FliA and the cytoplasmic anti‐sigma factor FlgM depresses transcription of class IV flagellar genes by inhibiting the FliA‐RNA polymerase association until completion of the hook‐basal body complex, at which point the anti‐sigma factor is secreted |
| GO:0016989 sigma factor antagonist activity | ISS file:PSEPK/flgM/flgM-uniprot.txt | NEW | Summary: FlgM directly antagonizes the flagellar sigma factor FliA, so sigma factor antagonist activity captures the direct molecular mechanism behind the transcriptional repression. Reason: GO:0016989 is defined as binding to a sigma factor and reducing its transcriptional activity, matching the conserved anti-sigma-28 function of FlgM. Supporting Evidence: file:PSEPK/flgM/flgM-uniprot.txt AltName: Full=Anti-sigma-28 factor file:PSEPK/flgM/flgM-uniprot.txt preventing expression of the flagellin genes file:PSEPK/flgM/flgM-uniprot.txt Anti-sigma factor FlgM file:PSEPK/flgM/flgM-deep-research-falcon.md FlgM is a **protein anti-sigma factor** that binds the flagellar sigma factor **FliA (σ28)** and inhibits FliA-dependent transcription of late/class III-IV flagellar genes until assembly reaches the proper checkpoint. file:PSEPK/flgM/flgM-deep-research-falcon.md whose primary molecular role is to **bind/sequester FliA**, preventing FliA from associating with RNA polymerase and thereby blocking FliA-dependent transcription until a defined assembly checkpoint is satisfied. PMID:27663176 FliA and the cytoplasmic anti‐sigma factor FlgM depresses transcription of class IV flagellar genes by inhibiting the FliA‐RNA polymerase association until completion of the hook‐basal body complex, at which point the anti‐sigma factor is secreted |
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Download this section (compressed HTML)Q: Which assembly checkpoint controls FlgM release or inactivation in P. putida KT2440?
Experiment: Measure FliA-dependent promoter activity and flagellin abundance in flgM deletion, fliA deletion, and assembly-defective backgrounds.
Hypothesis: FlgM restrains FliA-dependent flagellin transcription until the basal body-hook assembly checkpoint is satisfied.
Type: epistasis and promoter reporter assay
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