FlhB is an export-gate component whose cytoplasmic domain coordinates the change from rod/hook-type to filament-type substrate export with hook completion. In Pseudomonas putida KT2440 these functions are inferred from its conserved flagellar export-family identity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: flhB acts at the cytoplasmic-membrane export apparatus. Reason: The KT2440 assignment is inferred from the target-specific NCBIfam/InterPro classification and conserved export-system context; the mechanistic experiments cited here used Salmonella, not KT2440. The gate acts across the plasma membrane. Its assembly and detailed topology are distinguished from a generic membrane prediction. Supporting Evidence: PMID:29946050 FlhB undergoes autocleavage between N269 and P270, and the autocleavage is a critical event for the export specificity switch. file:PSEPK/flhB/flhB-uniprot.txt DR NCBIfam; TIGR00328; flhB; 1. |
| GO:0009306 protein secretion | IEA GO_REF:0000002 | MODIFY | Summary: flhB participates in flagellar type III protein export. Reason: The KT2440 assignment is inferred from the target-specific NCBIfam/InterPro classification and conserved export-system context; the mechanistic experiments cited here used Salmonella, not KT2440. FlhB is an export-gate component whose cytoplasmic domain coordinates the change from rod/hook-type to filament-type substrate export with hook completion. GO:0030254 is an is_a/part_of descendant of the existing broad process; it identifies the actual transport mechanism. Proposed replacements: protein secretion by the type III secretion system Supporting Evidence: PMID:29946050 FlhB undergoes autocleavage between N269 and P270, and the autocleavage is a critical event for the export specificity switch. file:PSEPK/flhB/flhB-uniprot.txt DR NCBIfam; TIGR00328; flhB; 1. |
| GO:0015031 protein transport | IEA GO_REF:0000002 | MODIFY | Summary: flhB participates in flagellar type III protein export. Reason: The KT2440 assignment is inferred from the target-specific NCBIfam/InterPro classification and conserved export-system context; the mechanistic experiments cited here used Salmonella, not KT2440. FlhB is an export-gate component whose cytoplasmic domain coordinates the change from rod/hook-type to filament-type substrate export with hook completion. GO:0030254 is an is_a/part_of descendant of the existing broad process; it identifies the actual transport mechanism. Proposed replacements: protein secretion by the type III secretion system Supporting Evidence: PMID:29946050 FlhB undergoes autocleavage between N269 and P270, and the autocleavage is a critical event for the export specificity switch. file:PSEPK/flhB/flhB-uniprot.txt DR NCBIfam; TIGR00328; flhB; 1. |
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: The membrane association is correctly localized more specifically to the plasma membrane. Reason: The KT2440 assignment is inferred from the target-specific NCBIfam/InterPro classification and conserved export-system context; the mechanistic experiments cited here used Salmonella, not KT2440. GO:0005886 is an is_a descendant of GO:0016020 and is already present. This is a specificity correction, not a reason to mark true membrane localization non-core. Proposed replacements: plasma membrane Supporting Evidence: PMID:29946050 FlhB undergoes autocleavage between N269 and P270, and the autocleavage is a critical event for the export specificity switch. file:PSEPK/flhB/flhB-uniprot.txt DR NCBIfam; TIGR00328; flhB; 1. |
| GO:0044780 bacterial-type flagellum assembly | IEA GO_REF:0000002 | ACCEPT | Summary: flhB performs a component step of flagellar construction. Reason: The KT2440 assignment is inferred from the target-specific NCBIfam/InterPro classification and conserved export-system context; the mechanistic experiments cited here used Salmonella, not KT2440. FlhB is an export-gate component whose cytoplasmic domain coordinates the change from rod/hook-type to filament-type substrate export with hook completion. This is direct machinery participation: it exports or guides structural substrates, rather than merely being required in a knockout experiment. Supporting Evidence: PMID:29946050 FlhB undergoes autocleavage between N269 and P270, and the autocleavage is a critical event for the export specificity switch. file:PSEPK/flhB/flhB-uniprot.txt DR NCBIfam; TIGR00328; flhB; 1. |
Loading supporting content…
Download this section (compressed HTML)Q: Which ATPase/gate stoichiometry and ion-coupling conditions operate in native KT2440, and how strongly do export bypass phenotypes transfer from Salmonella?
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)