fliI

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005524 ATP binding	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: FliI is a P-loop ATPase and binds ATP as its catalytic substrate. Reason: Correct but less informative than the catalytic activity annotation.
GO:0005737 cytoplasm	IEA GO_REF:0000120	ACCEPT	Summary: FliI is a peripheral cytoplasmic ATPase recruited to the export apparatus. Reason: Correct localization for the soluble catalytic component.
GO:0008564 protein-exporting ATPase activity	IEA GO_REF:0000003	ACCEPT	Summary: FliI couples ATP hydrolysis to handling of proteins exported by the flagellar apparatus. Reason: This is the most specific supported molecular function for FliI.
GO:0015986 proton motive force-driven ATP synthesis	IEA GO_REF:0000108	REMOVE	Summary: FliI consumes ATP during flagellar export and does not synthesize ATP from proton motive force. Reason: This erroneous inference confuses homology to rotary ATPases with FliI's export ATPase role. Supporting Evidence: file:PSEPK/fliI/fliI-deep-research-openscientist.md it consumes ATP to do work, rather than synthesizing it.
GO:0016887 ATP hydrolysis activity	IEA GO_REF:0000002	ACCEPT	Summary: ATP hydrolysis is the catalytic reaction performed by FliI. Reason: Correct catalytic activity, retained alongside the more contextual protein-exporting ATPase term. Supporting Evidence: file:PSEPK/fliI/fliI-deep-research-openscientist.md The core catalytic activity is ATP hydrolysis (ATP + H₂O → ADP + Pᵢ).
GO:0030254 protein secretion by the type III secretion system	IEA GO_REF:0000002	ACCEPT	Summary: FliI powers substrate handling in the flagellar type III export system. Reason: Correct process assignment for the flagellar export ATPase.
GO:0030257 type III protein secretion system complex	IEA GO_REF:0000002	ACCEPT	Summary: FliI associates with the cytoplasmic face of the flagellar type III export apparatus. Reason: Correct complex-level localization without assigning the whole apparatus activity to other subunits.
GO:0044780 bacterial-type flagellum assembly	IEA GO_REF:0000002	ACCEPT	Summary: FliI-dependent export supports ordered flagellar assembly. Reason: Core biological process for this dedicated export ATPase.
GO:0046933 proton-transporting ATP synthase activity, rotational mechanism	IEA GO_REF:0000118	REMOVE	Summary: FliI hydrolyzes ATP for protein export and is not a proton-transporting ATP synthase. Reason: Phylogenetic transfer across homologous ATPase families produced a contradicted molecular-function assignment. Supporting Evidence: file:PSEPK/fliI/fliI-deep-research-openscientist.md it consumes ATP to do work, rather than synthesizing it.
GO:0071973 bacterial-type flagellum-dependent cell motility	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: Functional flagellar export is required upstream for construction of a motile flagellum. Reason: Correct downstream phenotype, but assembly/export is the more direct process.