FrmC is a serine-dependent S-formylglutathione hydrolase that converts S-formylglutathione to formate and glutathione. It completes the glutathione-dependent formaldehyde detoxification route downstream of FrmA.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005829 cytosol | IEA GO_REF:0000118 | KEEP AS NON CORE | Summary: TreeGrafter assigns FrmC to the cytosol by phylogenetic inference. Reason: The localization is compatible with a soluble-metabolite hydrolase but lacks direct localization evidence in KT2440, so it should not define the core function. Supporting Evidence: file:PSEPK/frmC/frmC-uniprot.txt DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. |
| GO:0018738 S-formylglutathione hydrolase activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the direct catalytic function of FrmC. Reason: UniProt assigns EC 3.1.2.12, the FrmC-specific TIGR02821 and PTHR10061:SF1 families, and an intact Ser150-Asp228-His261 charge-relay system. Together these support an active S-formylglutathione hydrolase rather than a generic esterase or catalytically inactive family member. Supporting Evidence: file:PSEPK/frmC/frmC-uniprot.txt EC=3.1.2.12 file:PSEPK/frmC/frmC-deep-research-openscientist.md S-formylglutathione + H₂O → formate + GSH |
| GO:0046292 formaldehyde metabolic process | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Correct but broader than the formaldehyde catabolic process annotation. Reason: GO:0046292 is a true but redundant parent of the more specific GO:0046294 annotation. Supporting Evidence: file:PSEPK/frmC/frmC-uniprot.txt Serine hydrolase involved in the detoxification of formaldehyde |
| GO:0046294 formaldehyde catabolic process | IEA GO_REF:0000002 | ACCEPT | Summary: FrmC completes a glutathione-dependent formaldehyde catabolic branch. Reason: Hydrolysis of S-formylglutathione releases formate and regenerates glutathione. The KT2440 PP_1617 mutant phenotype independently supports a role in formaldehyde tolerance, without proving this exact reaction. Supporting Evidence: PMID:21261833 The second type consisted of mutants whose growth rates were at least twofold lower than that of the wild type (PP1617 |
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Download this section (compressed HTML)Q: How much formaldehyde flux passes through FrmA-FrmC versus the other KT2440 oxidation routes?
Experiment: Compare formaldehyde-to-formate flux in frmA, frmC, and alternative-dehydrogenase mutants.
Type: isotope-resolved formaldehyde flux analysis
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