galP encodes a porin-like outer-membrane protein associated with the gallate degradation cluster. It is probably involved in uptake of gallate or related pathway substrates at low environmental concentrations, but the transported substrate remains incompletely defined.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0015288 porin activity | IEA GO_REF:0000120 | ACCEPT | Summary: Porin activity is appropriate for GalP. Reason: UniProt names GalP as a porin-like protein and places it in the outer membrane porin family. Supporting Evidence: file:PSEPK/galP/galP-uniprot.txt Porin-like protein GalP file:PSEPK/galP/galP-uniprot.txt outer membrane porin file:PSEPK/galP/galP-goa.tsv GO:0015288 porin activity file:PSEPK/galP/galP-deep-research-falcon.md GalP is an **outer membrane porin** in the KT2440 **gallic acid (gallate) catabolic cluster** and is depicted as facilitating **entry of gallic acid into the periplasm** |
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: Generic membrane localization is correct but should be refined to cell outer membrane for this porin-like protein. Reason: GalP belongs to the outer membrane porin family; GO:0009279 is more specific than generic membrane for a Gram-negative bacterial porin. Proposed replacements: cell outer membrane Supporting Evidence: file:PSEPK/galP/galP-uniprot.txt outer membrane porin file:PSEPK/galP/galP-goa.tsv GO:0016020 membrane file:PSEPK/galP/galP-deep-research-falcon.md The KT2440 pathway schematic explicitly places GalP in the **outer membrane** |
| GO:0055085 transmembrane transport | IEA GO_REF:0000108 | ACCEPT | Summary: Transmembrane transport is plausible for GalP but remains broad because the transported substrate is not directly established. Reason: Porin activity implies transport across a membrane; UniProt supports a probable role in gallate pathway uptake. Supporting Evidence: file:PSEPK/galP/galP-uniprot.txt Probable transporter, possibly involved in the gallate file:PSEPK/galP/galP-goa.tsv GO:0055085 transmembrane transport file:PSEPK/galP/galP-deep-research-falcon.md recent primary literature specifically places **galP** in the **gallic acid (gallate) catabolic gene cluster**, where it is annotated as an **outer membrane porin** involved in gallate/gallic-acid utilization |
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Download this section (compressed HTML)Q: Does GalP transport gallate directly, or does it transport another gal-cluster intermediate or aromatic acid?
Q: Is GalP-mediated outer-membrane uptake essential, or is it redundant with other aromatic-acid transport systems (e.g. protocatechuate-related PcaK), given that strains lacking galT and galP still release gallic acid?
Experiment: Measure gallate uptake in galP mutant and complemented strains at low gallate concentrations and test purified GalP channel selectivity in reconstituted membranes.
Type: transport substrate specificity assay
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