gapB (PP_2149) encodes a type I glyceraldehyde-3-phosphate dehydrogenase family enzyme. It is predicted to catalyze the NAD+-dependent phosphorylating oxidation of D-glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, a lower central-carbon reaction shared by glycolytic and gluconeogenic flux. It is a plausible alternate or condition-specific GAPDH relative to the already curated gapA enzyme.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | IEA GO_REF:0000003 | ACCEPT | Summary: glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity is consistent with the curated UniProt name, EC/family evidence, and the gene product role summarized here. Reason: This is a specific, biologically appropriate annotation for this gene product. |
| GO:0006006 glucose metabolic process | IEA GO_REF:0000002 | ACCEPT | Summary: glucose metabolic process is consistent with the curated UniProt name, EC/family evidence, and the gene product role summarized here. Reason: This is a specific, biologically appropriate annotation for this gene product. |
| GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor | IEA GO_REF:0000002 | MODIFY | Summary: oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor captures the broad idea but is less precise than glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity for this protein. Reason: Replace or supplement this broad term with GO:0004365 (glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity) based on the UniProt family and reaction evidence. Proposed replacements: glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| GO:0050661 NADP binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: NADP binding cannot be confidently ruled out for gapB. UniProt lists gapB as EC 1.2.1.- with cofactor undetermined, and P. putida is known to carry both NAD- and NADP-dependent GAPDH isoforms, so removing the propagated NADP binding annotation while keeping NAD binding could be exactly backwards. Reason: Cofactor specificity is not established for this entry; retain the propagated NADP binding annotation as non-core rather than removing it. |
| GO:0051287 NAD binding | IEA GO_REF:0000002 | ACCEPT | Summary: NAD binding is consistent with the curated UniProt name, EC/family evidence, and the gene product role summarized here. Reason: This is a specific, biologically appropriate annotation for this gene product. |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)