Summary: Generic catalytic activity is true but uninformative beside the two separately annotated GatABC synthase activities.

Reason: GO:0050566 and GO:0050567 already capture the Asn- and Gln-tRNA transamidation reactions specifically.

Summary: GatB supports translation, but its direct role is tRNA transamidation.

Reason: Translation is a downstream context broader than either GatABC charging reaction.

Summary: Generic ligase activity is correct but uninformative beside the two separately annotated GatABC synthase activities.

Reason: GO:0050566 and GO:0050567 already capture ATP-dependent ligation on the two tRNA-bound substrates.

Summary: The broad amido-N-donor ligase term is true but uninformative beside the two tRNA-specific activities.

Reason: GO:0050566 and GO:0050567 already capture the exact Asn-tRNA(Asn) and Gln-tRNA(Gln) products.

Summary: GatB is a required subunit of heterotrimeric GatABC.

Reason: UniProt explicitly specifies the A/B/C heterotrimer.

Summary: GatABC can form Asn-tRNA(Asn) by transamidating Asp-tRNA(Asn).

Reason: The reviewed entry includes the exact asparaginyl-tRNA synthase reaction, but GatB contributes to rather than independently enables the whole-complex activity.

Summary: GatABC can form Gln-tRNA(Gln) by transamidating Glu-tRNA(Gln).

Reason: The reviewed entry includes the exact glutaminyl-tRNA synthase reaction, but GatB contributes to rather than independently enables the whole-complex activity.

Summary: GatABC has the reaction capacity, but this route is not established in KT2440.

Reason: The reaction requires Glu-tRNA(Gln), but KT2440 has direct GlnRS and no evidence establishes that its GltX supplies this misacylated precursor. The characterized Pseudomonas aeruginosa system instead uses discriminating GluRS and direct GlnRS.

Summary: GatB participates directly in indirect Asn-tRNA(Asn) formation from Asp-tRNA(Asn).

Reason: The KT2440 GatB record contains the exact asparaginyl-tRNA synthase reaction, and the corresponding ND-AspRS/GatABC route is experimentally established in Pseudomonas aeruginosa. This row originally proposed GO:0070680 asparaginyl-tRNAAsn biosynthesis via transamidation, which is obsolete in the GO release 2026-07-26. Its replaced_by, GO:0070981 L-asparagine biosynthetic process, is not used because the indirect route ends at Asn-tRNA(Asn), not free asparagine, so GO:0043039 tRNA aminoacylation (the is_a parent of the obsolete term) is asserted instead. The GOA descendant GO:0070681 (glutaminyl-tRNAGln biosynthesis via transamidation) does not make this row redundant: that row is UNDECIDED here because the Gln transamidation route is not established in KT2440, whereas this row rests on the Asn route.