Summary: GatC supports translation through tRNA transamidation, but translation is downstream and broad.

Reason: Its direct role is structural participation in GatABC-dependent tRNA charging.

Summary: Correct charging is a consequence of GatABC transamidation, not a distinct GatC fidelity-regulatory activity.

Reason: Correct amino acid identity is a consequence of GatABC transamidation rather than a separate GatC regulatory activity.

Summary: GatABC has the reaction capacity, but this route is not established in KT2440.

Reason: UniProt records the Glu-tRNA(Gln) reaction for the A/B/C heterotrimer, but KT2440 has direct GlnRS and no evidence establishes production of the required Glu-tRNA(Gln) precursor by its GltX. The characterized Pseudomonas aeruginosa system instead uses discriminating GluRS.

Summary: GatC is explicitly identified as the C subunit of heterotrimeric GatABC.

Reason: This missing complex annotation captures GatC's direct structural role more precisely than the broad translation and fidelity annotations.

Summary: GatC contributes structurally to GatABC-dependent conversion of Asp-tRNA(Asn) to Asn-tRNA(Asn).

Reason: The KT2440 GatC record includes the exact asparaginyl-tRNA synthase reaction, while comparative experiments establish the indirect Pseudomonas Asn route.

Summary: GatC is required in GatABC for indirect Asn-tRNA(Asn) biosynthesis via transamidation.

Reason: KT2440 lacks a canonical AsnRS, encodes non-discriminating AspS, and carries GatABC with the exact Asn-forming reaction documented for GatC. This row originally proposed GO:0070680 asparaginyl-tRNAAsn biosynthesis via transamidation, which is obsolete in the GO release 2026-07-26. Its replaced_by, GO:0070981 L-asparagine biosynthetic process, is not used because the indirect route ends at Asn-tRNA(Asn), not free asparagine, so GO:0043039 tRNA aminoacylation (the is_a parent of the obsolete term) is asserted instead. The GOA descendant GO:0070681 (glutaminyl-tRNAGln biosynthesis via transamidation) does not make this row redundant: that row is UNDECIDED here because the Gln transamidation route is not established in KT2440, whereas this row rests on the Asn route.