gcvH2

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005737 cytoplasm	IEA GO_REF:0000118	MARK AS OVER ANNOTATED	Summary: Cytoplasm is a generic parent of the more informative cytosol annotation already present. Reason: Retain GO:0005829 as non-core location context instead of duplicating it with its parent.
GO:0005829 cytosol	IEA GO_REF:0000118	KEEP AS NON CORE	Summary: A soluble cytosolic location is plausible for the bacterial carrier but is not a core function. Reason: Retain the more specific of the two generic location annotations outside the core summary.
GO:0005960 glycine cleavage complex	IEA GO_REF:0000002	ACCEPT	Summary: GcvH2 is the lipoyl-bearing carrier component of the four-protein system. Reason: The reviewed UniProt record explicitly identifies P, H, T, and L proteins as system components. Supporting Evidence: file:PSEPK/gcvH2/gcvH2-uniprot.txt The glycine cleavage system is composed of four proteins: P,
GO:0009249 protein lipoylation	IEA GO_REF:0000118	UNDECIDED	Summary: GcvH2 is a lipoylated carrier, but its precise role in the KT2440 lipoyl-transfer route has not been established. Reason: Participation in a biological process does not require catalysis, and GcvH proteins can be obligatory intermediates in bacterial lipoyl relays. The covalent carrier state is certain, but available KT2440 evidence does not distinguish an active relay role from being only a LipA/LipB substrate.
GO:0019464 glycine decarboxylation via glycine cleavage system	IEA GO_REF:0000120	ACCEPT	Summary: Intermediate shuttling by GcvH2 is required for this exact process. Reason: UniProt describes transfer of the methylamine group from P protein to T protein on GcvH2. Supporting Evidence: file:PSEPK/gcvH2/gcvH2-uniprot.txt The H protein shuttles the methylamine group of glycine from
GO:0031405 lipoic acid binding	ISS file:PSEPK/gcvH2/gcvH2-uniprot.txt	NEW	Summary: This captures the covalently lipoylated carrier function omitted from current GOA. Reason: The reviewed HAMAP-backed record states that GcvH2 binds one lipoyl cofactor covalently. Supporting Evidence: file:PSEPK/gcvH2/gcvH2-uniprot.txt Binds 1 lipoyl cofactor covalently.