GlnS is the class I glutamine--tRNA ligase of Pseudomonas putida KT2440. It uses ATP to activate L-glutamine and transfers it directly to tRNA(Gln), producing Gln-tRNA(Gln) for translation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000166 nucleotide binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Nucleotide binding is true but uninformative beside the separately annotated ATP binding term. Reason: GO:0005524 already captures the ATP substrate interaction specifically. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0004812 aminoacyl-tRNA ligase activity | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: Generic aminoacyl-tRNA ligase activity is true but uninformative beside the separately annotated GlnRS activity. Reason: GO:0004819 already captures the glutamine- and tRNA(Gln)-specific reaction. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt RecName: Full=Glutamine--tRNA ligase |
| GO:0004819 glutamine-tRNA ligase activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the substrate-specific catalytic activity of GlnS. Reason: UniProt assigns the direct tRNA(Gln), L-glutamine, and ATP reaction. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0005524 ATP binding | IEA GO_REF:0000120 | ACCEPT | Summary: ATP is the activating substrate in the GlnRS reaction. Reason: The catalytic record consumes ATP and produces AMP plus diphosphate. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Cytoplasm is a correct broad parent of the more informative cytosol annotation. Reason: The reviewed entry supports a soluble intracellular location, while GO:0005829 records it more specifically. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm |
| GO:0005829 cytosol | IEA GO_REF:0000118 | ACCEPT | Summary: Cytosol is consistent with the reviewed cytoplasmic localization. Reason: The bacterial soluble compartment is supported by UniProt; no membrane or export feature is present. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm |
| GO:0006412 translation | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: GlnS supports translation by supplying Gln-tRNA(Gln), but translation is broader than its direct process. Reason: The immediate role is glutaminyl-tRNA aminoacylation; translation is a downstream process context. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0006418 tRNA aminoacylation for protein translation | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: Translational tRNA charging is true but uninformative beside the separately annotated glutaminyl-tRNA process. Reason: GO:0006425 already captures the direct substrate-specific process. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0006424 glutamyl-tRNA aminoacylation | IEA GO_REF:0000104 | REMOVE | Summary: GlnS does not catalyze glutamyl-tRNA aminoacylation. Reason: The exact reviewed reaction uses L-glutamine and tRNA(Gln), not L-glutamate and tRNA(Glu); this electronic transfer conflicts with the substrate-specific record. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0006425 glutaminyl-tRNA aminoacylation | IEA GO_REF:0000120 | ACCEPT | Summary: GlnS directly forms Gln-tRNA(Gln). Reason: The catalytic reaction exactly matches glutaminyl-tRNA aminoacylation. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
| GO:0043039 tRNA aminoacylation | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Broad tRNA aminoacylation is true but uninformative beside the separately annotated glutaminyl-tRNA process. Reason: GO:0006425 already captures the direct GlnRS process specifically. Supporting Evidence: file:PSEPK/glnS/glnS-uniprot.txt Reaction=tRNA(Gln) + L-glutamine + ATP |
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