GltX is the class I glutamate--tRNA ligase of Pseudomonas putida KT2440. It uses ATP to attach L-glutamate to tRNA(Glu), producing Glu-tRNA(Glu) for translation. It is not known whether it also misacylates tRNA(Gln) in this GlnRS-containing strain.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000049 tRNA binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: tRNA binding is inherent to the documented GltX reaction but is less informative than catalysis. Reason: The reaction explicitly uses tRNA(Glu); GO:0004818 captures the core activity. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Reaction=tRNA(Glu) + L-glutamate + ATP |
| GO:0000166 nucleotide binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Nucleotide binding is true but uninformative beside the separately annotated ATP binding activity. Reason: The exact reaction and annotated binding site identify ATP, which is already captured by GO:0005524. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Reaction=tRNA(Glu) + L-glutamate + ATP |
| GO:0004812 aminoacyl-tRNA ligase activity | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: Generic aminoacyl-tRNA ligase activity is true but uninformative beside the substrate-specific annotation. Reason: GO:0004818 already captures the EC 6.1.1.17 reaction specifically. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt RecName: Full=Glutamate--tRNA ligase |
| GO:0004818 glutamate-tRNA ligase activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the substrate-specific catalytic activity of GltX. Reason: The reviewed record directly assigns tRNA(Glu), L-glutamate, and ATP as substrates. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Reaction=tRNA(Glu) + L-glutamate + ATP |
| GO:0005524 ATP binding | IEA GO_REF:0000120 | ACCEPT | Summary: ATP is the activating substrate for glutamyl-tRNA formation. Reason: UniProt records both ATP consumption and a specific ATP-binding site. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Reaction=tRNA(Glu) + L-glutamate + ATP |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Cytoplasm is correct but broader than the separately annotated cytosol location for this soluble enzyme. Reason: The reviewed entry assigns GltX to the cytoplasm, while GO:0005829 more precisely captures the soluble compartment used in the core function. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm |
| GO:0005829 cytosol | IEA GO_REF:0000118 | ACCEPT | Summary: Cytosol is consistent with the reviewed cytoplasmic localization. Reason: The protein is a soluble monomer with no membrane or export feature. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm |
| GO:0006418 tRNA aminoacylation for protein translation | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: The broad translational charging term is true but uninformative beside the substrate-specific process annotation. Reason: GO:0006424 already captures the defined glutamate and tRNA(Glu) substrates. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Catalyzes the attachment of glutamate to tRNA(Glu) |
| GO:0006424 glutamyl-tRNA aminoacylation | IEA GO_REF:0000120 | ACCEPT | Summary: GltX directly produces Glu-tRNA(Glu). Reason: The assigned reaction exactly matches glutamyl-tRNA aminoacylation. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Catalyzes the attachment of glutamate to tRNA(Glu) |
| GO:0008270 zinc ion binding | IEA GO_REF:0000002 | REMOVE | Summary: The zinc-binding call is contradicted by loss of the conserved four-cysteine zinc-binding cluster. Reason: Alignment to zinc-binding Escherichia coli GluRS P04805 shows that Q88LF6 retains the first Cys pair but lacks the downstream cysteine needed to preserve the four-cysteine zinc-binding cluster. The InterPro-derived zinc-binding transfer is therefore not appropriate for this protein. Supporting Evidence: file:PSEPK/gltX/gltX-bioinformatics/RESULTS.md Q88LF6 therefore does not preserve the four-cysteine zinc-binding cluster present in P04805. |
| GO:0043039 tRNA aminoacylation | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: The broad tRNA aminoacylation term is true but uninformative beside the substrate-specific process annotation. Reason: GO:0006424 already captures the direct GltX process specifically. Supporting Evidence: file:PSEPK/gltX/gltX-uniprot.txt Catalyzes the attachment of glutamate to tRNA(Glu) |
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Download this section (compressed HTML)Q: Does Q88LF6 also recognize tRNA(Gln) and generate Glu-tRNA(Gln) under any physiological condition despite the presence of GlnS?
Experiment: Compare purified GltX aminoacylation kinetics with native KT2440 tRNA(Glu) and tRNA(Gln), and identify charged products by acid-urea electrophoresis or LC-MS.
Hypothesis: Q88LF6 may discriminate strongly against tRNA(Gln) in this GlnRS-containing strain.
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