gntZ (PP_4043) encodes a 6-phosphogluconate dehydrogenase family enzyme in Pseudomonas putida KT2440. UniProt and GOA identify it as a decarboxylating 6-phosphogluconate dehydrogenase (EC 1.1.1.44) with NADP binding, placing it in the pentose phosphate pathway step that oxidatively decarboxylates 6-phosphogluconate to ribulose 5-phosphate while generating NADPH. Current evidence supports Q88FP6 as the single KT2440 6-phosphogluconate dehydrogenase, acting at the 6-phosphogluconate branch point between Entner-Doudoroff flux and the oxidative PPP.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004616 phosphogluconate dehydrogenase (decarboxylating) activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the exact catalytic activity for the annotated EC 1.1.1.44 enzyme. UniProt names Q88FP6 as decarboxylating 6-phosphogluconate dehydrogenase, and GOA links the term to InterPro and EC evidence. Reason: The term captures the core molecular function of gntZ in the oxidative pentose phosphate pathway. Support is family-level: Q88FP6 matches the 6-phosphogluconate dehydrogenase signatures InterPro IPR006183 (Pgluconate_DH), Pfam PF00393 (6PGD C-terminal domain) and the NADP-binding domain IPR006115, and NCBIfam TIGR00872 (gnd_rel, the Gnd-related decarboxylating 6PGDH clade). The submitter-supplied protein name is not used as support, and Q88FP6 has not been assayed directly. Supporting Evidence: file:PSEPK/gntZ/gntZ-uniprot.txt DR InterPro; IPR006183; Pgluconate_DH. file:PSEPK/gntZ/gntZ-uniprot.txt DR NCBIfam; TIGR00872; gnd_rel; 1. file:PSEPK/gntZ/gntZ-uniprot.txt DR Pfam; PF00393; 6PGD; 1. |
| GO:0006098 pentose-phosphate shunt | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: The enzyme catalyzes the oxidative PPP decarboxylation step producing ribulose 5-phosphate, so the broad pentose-phosphate shunt annotation is correct but less informative than the oxidative-branch child term. Reason: The pathway placement follows both the enzyme chemistry and the UniPathway mapping in the GOA record; GO:0009051 is used for the core process. Supporting Evidence: file:PSEPK/gntZ/gntZ-uniprot.txt CC -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway. file:PSEPK/gntZ/gntZ-deep-research-openscientist.md This is the committed oxidative-decarboxylating step of the oxidative pentose phosphate pathway (oxPPP). |
| GO:0009051 pentose-phosphate shunt, oxidative branch | ISS file:PSEPK/gntZ/gntZ-deep-research-openscientist.md | NEW | Summary: GntZ performs the oxidative decarboxylation that completes the oxidative pentose-phosphate branch and produces ribulose 5-phosphate and NADPH. Reason: This child term captures the demonstrated pathway placement more precisely than the existing general pentose-phosphate shunt annotation. Supporting Evidence: file:PSEPK/gntZ/gntZ-deep-research-openscientist.md This is the committed oxidative-decarboxylating step of the oxidative pentose phosphate pathway (oxPPP). |
| GO:0050661 NADP binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: NADP binding is consistent with the dehydrogenase family and with the NADP-dependent oxidative PPP reaction, but it is a cofactor property rather than the most informative function term. Reason: Keep as useful catalytic context while treating GO:0004616 as the core molecular function. Supporting Evidence: file:PSEPK/gntZ/gntZ-deep-research-openscientist.md The enzyme catalyzes the NADP⁺-dependent oxidative decarboxylation of 6-phospho-D-gluconate to D-ribulose-5-phosphate, CO₂, and NADPH |
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