HbdH is a predicted soluble D-3-hydroxybutyrate dehydrogenase. Its EC and family assignments support oxidation of D-3-hydroxybutyrate to acetoacetate, while its precise contribution to this reaction in KT2440 remains untested.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003858 3-hydroxybutyrate dehydrogenase activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the specific EC- and family-supported HbdH reaction. Reason: Family-level support. Q88IC6 matches the substrate-specific InterPro 3-hydroxybutyrate dehydrogenase family (IPR011294) and the NCBIfam TIGR01963 PHB_DH model within the SDR family. The UniProt name and EC 1.1.1.30 are submitter-imported (ECO:0000313) and are not cited. A D-3-hydroxybutyrate dehydrogenase from P. putida has been crystallized with NAD+ (PMID:17958702), but the cached abstract does not state the strain, so it corroborates the family rather than this accession. No assay on PP_3073 itself is cached. Supporting Evidence: file:PSEPK/hbdH/hbdH-uniprot.txt IPR011294; 3-OHbutyrate_DH. file:PSEPK/hbdH/hbdH-uniprot.txt TIGR01963; PHB_DH; 1. |
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Download this section (compressed HTML)Q: What are the cofactor preference and in vivo reaction direction of KT2440 HbdH?
Experiment: Purify Q88IC6 and measure reversible D-3-hydroxybutyrate/acetoacetate kinetics with NAD(H) and NADP(H), then test growth of an hbdH deletion on D-3-hydroxybutyrate.
Type: enzyme kinetics and carbon-source growth assay
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