hemE

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004853 uroporphyrinogen decarboxylase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is the exact substrate-level activity of HemE. Reason: UniProt assigns EC 4.1.1.37 and describes four decarboxylations yielding coproporphyrinogen III. Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt Catalyzes the decarboxylation of four acetate groups of
GO:0005737 cytoplasm	IEA GO_REF:0000120	MARK AS OVER ANNOTATED	Summary: Cytoplasm is compatible with HemE biology but is the broader parent of the separately annotated cytosol term. Reason: The more specific cytosol annotation is also present and supported for this soluble enzyme, making cytoplasm redundant as a distinct retained annotation. Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm
GO:0005829 cytosol	IEA GO_REF:0000118	ACCEPT	Summary: Cytosol is the more specific informative location for the soluble bacterial HemE enzyme. Reason: The TreeGrafter cytosol call is consistent with UniProt's cytoplasm assignment and avoids retaining both a child term and its broader parent as equivalent annotations. Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm
GO:0006778 porphyrin-containing compound metabolic process	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This broad metabolic-process annotation is correct but not core. Reason: HemE does participate in porphyrin-containing compound metabolism, but its substrate-specific decarboxylase activity and biosynthetic process assignments describe the role more informatively. Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
GO:0006779 porphyrin-containing compound biosynthetic process	IEA GO_REF:0000002	MODIFY	Summary: The biosynthetic assignment is sound and can be made endpoint-specific for KT2440. Reason: In KT2440, HemE supplies coproporphyrinogen III to the HemF/HemN, protoporphyrinogen oxidase, and ferrochelatase reactions that produce heme B, supporting the live endpoint term GO:0006785. Proposed replacements: heme B biosynthetic process Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt uroporphyrinogen-III to yield coproporphyrinogen-III.
GO:0006783 heme biosynthetic process	IEA GO_REF:0000118	ACCEPT	Summary: HemE is an obligatory shared enzyme in protoporphyrin-dependent heme-b synthesis. Reason: Coproporphyrinogen III is required for the subsequent HemF/HemN, protoporphyrinogen oxidase, and ferrochelatase reactions. Supporting Evidence: file:PSEPK/hemE/hemE-uniprot.txt PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX