hemH

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004325 protoporphyrin ferrochelatase activity	IEA GO_REF:0000120	ACCEPT	Summary: This term precisely describes the terminal HemH reaction. Reason: UniProt assigns EC 4.98.1.1 and explicitly describes ferrous insertion into protoporphyrin IX. Supporting Evidence: file:PSEPK/hemH/hemH-uniprot.txt Catalyzes the ferrous insertion into protoporphyrin IX.
GO:0005737 cytoplasm	IEA GO_REF:0000120	ACCEPT	Summary: Cytoplasmic localization is explicitly predicted for the KT2440 enzyme. Reason: UniProt assigns HemH to the cytoplasm and does not predict a membrane anchor. Supporting Evidence: file:PSEPK/hemH/hemH-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm
GO:0006779 porphyrin-containing compound biosynthetic process	IEA GO_REF:0000104	MODIFY	Summary: The broad porphyrin process is correct but can be replaced by the exact product process. Reason: HemH directly produces heme B, so GO:0006785 is a supported live replacement for this broad porphyrin-biosynthesis annotation. Proposed replacements: heme B biosynthetic process Supporting Evidence: file:PSEPK/hemH/hemH-uniprot.txt biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
GO:0006783 heme biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: HemH directly performs the final heme-b biosynthetic step. Reason: Insertion of Fe2+ into protoporphyrin IX creates heme b, so the broader heme-biosynthesis row is correct. GO:0006785 captures the endpoint more specifically in the replacement and core-function records. Supporting Evidence: file:PSEPK/hemH/hemH-uniprot.txt biosynthesis; protoheme from protoporphyrin-IX: step 1/1.