IlvD is the iron-sulfur-dependent dihydroxy-acid dehydratase that dehydrates the IlvC products in both valine and isoleucine biosynthesis. It forms 2-oxoisovalerate or 3-methyl-2-oxopentanoate for terminal transamination by IlvE.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000287 magnesium ion binding | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: Magnesium binding is a secondary cofactor annotation. Reason: The reviewed UniProt record includes a magnesium cofactor assignment. |
| GO:0003824 catalytic activity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: This generic term is redundant with dihydroxy-acid dehydratase activity. Reason: GO:0004160 specifies the actual reaction. |
| GO:0004160 dihydroxy-acid dehydratase activity | IEA GO_REF:0000120 | ACCEPT | Summary: This is the specific IlvD catalytic function. Reason: EC 4.2.1.9 and the IlvD-specific HAMAP/NCBIfam assignments support it. Supporting Evidence: file:PSEPK/ilvD/ilvD-uniprot.txt Full=Dihydroxy-acid dehydratase |
| GO:0005829 cytosol | IEA GO_REF:0000118 | KEEP AS NON CORE | Summary: Cytosol is plausible for this soluble bacterial pathway enzyme. Reason: Location is compatible but not the defining function. |
| GO:0009082 branched-chain amino acid biosynthetic process | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: This broad process is redundant with valine and isoleucine branch calls. Reason: IlvD has explicit pathway placement in both branches. |
| GO:0009099 L-valine biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: IlvD catalyzes step 3 of valine biosynthesis. Reason: The reviewed UniProt pathway record explicitly assigns this role. |
| GO:0016836 hydro-lyase activity | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: This broad chemistry term is redundant with GO:0004160. Reason: The exact dihydroxy-acid substrate is already represented. |
| GO:0051537 2 iron, 2 sulfur cluster binding | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: The catalytic 2Fe-2S cluster is valid mechanistic information. Reason: UniProt assigns a single 2Fe-2S cluster to the IlvD family. |
| GO:1901705 L-isoleucine biosynthetic process | IEA file:PSEPK/ilvD/ilvD-uniprot.txt | NEW | Summary: IlvD also catalyzes step 3 of isoleucine biosynthesis. Reason: The reviewed UniProt pathway record explicitly assigns this branch. |
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Download this section (compressed HTML)The gene-level OpenScientist request for PSEPK IlvD (Q88CQ2/PP_5128)
reached the provider's 3600-second service ceiling and was cancelled without
producing a report. No provider-named deep-research file was created. This
first-pass review therefore remains grounded in the exact UniProt reaction,
cofactor, and family record plus the successful module/pathway/taxon
OpenScientist report for branched-chain amino-acid biosynthesis.
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