Ivd is an FAD-dependent 3-methylbutanoyl-CoA (isovaleryl-CoA) dehydrogenase that forms 3-methylcrotonyl-CoA in the distal L-leucine catabolic pathway. It belongs to the acyl-CoA dehydrogenase family and transfers reducing equivalents onward through electron-transfer flavoprotein.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003995 acyl-CoA dehydrogenase activity | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Correct family-level activity retained as non-core beside the substrate-specific term. Reason: Ivd is an acyl-CoA dehydrogenase, while GO:0008470 captures its informative physiological substrate. |
| GO:0006552 L-leucine catabolic process | IEA GO_REF:0000118 | ACCEPT | Summary: Correct distal L-leucine catabolic pathway assignment. Reason: Ivd converts leucine-derived 3-methylbutanoyl-CoA to 3-methylcrotonyl-CoA. |
| GO:0008470 3-methylbutanoyl-CoA dehydrogenase activity | IEA GO_REF:0000003 | ACCEPT | Summary: Correct enzyme-specific molecular function. Reason: The exact Ivd product assignment and EC 1.3.8.4 support this activity. |
| GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Mechanistically correct oxidoreductase parent term. Reason: Retain the valid parent chemistry as non-core; the substrate-specific dehydrogenase term is substantially more informative. |
| GO:0016937 short-chain fatty acyl-CoA dehydrogenase activity | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: The close LiuA ortholog also oxidizes butyryl-CoA, but this is a minor promiscuous activity beside its preferred isovaleryl-CoA reaction. Reason: Do not treat the short-chain activity as the physiological core function; retain the exact 3-methylbutanoyl-CoA dehydrogenase activity as core. Supporting Evidence: PMID:18625020 and butyryl-CoA as substrates. |
| GO:0050660 flavin adenine dinucleotide binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: FAD is a required cofactor but binding alone is not the core function. Reason: Retain the genuine cofactor-binding function as non-core beside the catalytic activity. |
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Download this section (compressed HTML)Experiment: Assay purified Ivd with 3-methylbutanoyl-CoA and electron-transfer flavoprotein, and test an ivd deletion for growth on leucine or isovalerate as carbon sources.
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