Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000166 nucleotide binding	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: True but too broad relative to ATP binding and ATP hydrolysis.
GO:0000287 magnesium ion binding	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: Magnesium supports the ATPase reaction but is not the defining core function.
GO:0005524 ATP binding	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct substrate binding for the P-type ATPase cycle.
GO:0005886 plasma membrane	IEA GO_REF:0000120	ACCEPT	Summary: Correct location for the integral KdpB ATPase subunit.
GO:0006813 potassium ion transport	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct process, though the transmembrane child term is more precise.
GO:0008556 P-type potassium transmembrane transporter activity	IEA GO_REF:0000120	ACCEPT	Summary: The term correctly describes the collective KdpFABC pump activity, but KdpB supplies ATP-dependent energy coupling while KdpA supplies the potassium-selective pathway. Reason: The term is correct in substance. The qualifier should ideally be contributes_to rather than enables because KdpB independently supplies ATP hydrolysis, while the complete transporter activity belongs to the heteromeric pump; core_functions records both relationships.
GO:0016020 membrane	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct but less precise than plasma membrane.
GO:0016887 ATP hydrolysis activity	IEA GO_REF:0000002	ACCEPT	Summary: Direct catalytic activity of KdpB.
GO:0071805 potassium ion transmembrane transport	IEA GO_REF:0000108	ACCEPT	Summary: Directly captures the transport process energized by KdpB.

Core Functions

Hydrolyzes ATP through a P-type ATPase cycle to energize KdpA-mediated potassium uptake.

Molecular Function:

ATP hydrolysis activity

Directly Involved In:

potassium ion transmembrane transport

Cellular Locations:

plasma membrane

Supporting Evidence:

file:PSEPK/kdpB/kdpB-uniprot.txt
PMID:28636601

References

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Suggested Experiments

Experiment: Reconstitute KdpABCF and quantify ATPase-to-potassium coupling across subunit variants.

Type: membrane-pump reconstitution

📚 Additional Documentation

Notes

(kdpB-notes.md)

kdpB curation notes

Evidence assessment

PSEPK A0A140FWL1 is the P-type ATPase subunit of KdpFABC. Structural work on the
E. coli ortholog establishes the division of labor in which KdpA handles the
potassium pathway and ATP energy is harnessed by KdpB [PMID:28636601, "the
energy of ATP is harnessed by KdpB"]. UniProt independently identifies the
P-type ATPase family, ATP-binding region, and catalytic phosphorylation cycle
[file:PSEPK/kdpB/kdpB-uniprot.txt].

Curation decision

ATP hydrolysis activity (GO:0016887) is KdpB's direct catalytic molecular
function. P-type potassium transmembrane transporter activity (GO:0008556) is a
property of the assembled pump, so KdpB contributes to it but does not provide
the complete activity independently.

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