Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000155 phosphorelay sensor kinase activity	IEA GO_REF:0000120	ACCEPT	Summary: Specific core signaling activity of KdpD.
GO:0000160 phosphorelay signal transduction system	IEA GO_REF:0000002	ACCEPT	Summary: Directly captures KdpD-to-KdpE two-component signaling.
GO:0004673 protein histidine kinase activity	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct parent activity, but phosphorelay sensor kinase activity is more informative.
GO:0005737 cytoplasm	IEA GO_REF:0000117	REMOVE	Summary: KdpD is an integral membrane sensor with cytoplasmic signaling domains; domain topology does not make the whole protein a cytoplasmic gene product. Reason: The UniProt record places full-length KdpD in the membrane as a multipass membrane protein. Its large cytosolic input and transmitter domains explain the inference but do not make the whole protein a cytoplasmic gene product, so the ARBA assignment is a localization overreach. Supporting Evidence: file:PSEPK/kdpD/kdpD-uniprot.txt SUBCELLULAR LOCATION: Membrane
GO:0005886 plasma membrane	IEA GO_REF:0000118	ACCEPT	Summary: Correct location for the KdpD sensor kinase.
GO:0007165 signal transduction	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: Correct but broad relative to phosphorelay signal transduction.
GO:0016020 membrane	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Correct but less precise than plasma membrane.
GO:0016301 kinase activity	IEA GO_REF:0000104	MARK AS OVER ANNOTATED	Summary: Too broad relative to phosphorelay sensor kinase activity.
GO:0016740 transferase activity	IEA GO_REF:0000104	MARK AS OVER ANNOTATED	Summary: Too broad relative to phosphorelay sensor kinase activity.
GO:0016772 transferase activity, transferring phosphorus-containing groups	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: Too broad relative to phosphorelay sensor kinase activity.

Core Functions

Senses Kdp-inducing conditions and phosphorylates KdpE in a membrane-associated phosphorelay.

Molecular Function:

phosphorelay sensor kinase activity

Directly Involved In:

phosphorelay signal transduction system

Cellular Locations:

plasma membrane

Supporting Evidence:

file:PSEPK/kdpD/kdpD-uniprot.txt
PMID:14534307

References

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Suggested Experiments

Experiment: Measure KdpD autophosphorylation and KdpE transfer across potassium and osmotic conditions.

Type: phosphorelay assay

📚 Additional Documentation

Notes

(kdpD-notes.md)

kdpD curation notes

Evidence assessment

KdpD is a membrane sensor kinase with large cytosolic input and transmitter
domains. E. coli experiments establish that membrane-bound KdpD controls
KdpE-dependent kdpFABC expression and describe four transmembrane segments plus
cytoplasmic domains [PMID:14534307, "The membrane-bound sensor kinase KdpD
consists of an N-terminal input domain"]. UniProt predicts the corresponding
multi-pass topology for PSEPK Q88FD9 [file:PSEPK/kdpD/kdpD-uniprot.txt].

Curation decision

The full-length protein is assigned to the plasma membrane. The ARBA cytoplasm
annotation likely reflects its extensive cytosolic domains, but located_in cytoplasm misstates the location of the integral membrane gene product and is
therefore removed.

📄 View Raw YAML