liuC

UniProt ID: Q88FM3
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

LiuC is a bacterial methylglutaconyl-CoA hydratase that converts 3-methylglutaconyl-CoA to (S)-3-hydroxy-3-methylglutaryl-CoA in the distal L-leucine/isovalerate catabolic pathway.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0003824 catalytic activity
IEA
GO_REF:0000120 		MODIFY
Summary: Catalytic activity is uninformative beside the exact hydratase term.
Reason: Replace the generic catalytic term with the substrate-specific hydratase activity.
GO:0004490 methylglutaconyl-CoA hydratase activity
IEA
GO_REF:0000003 		ACCEPT
Summary: Correct enzyme-specific molecular function.
Reason: The exact LiuC product assignment and EC 4.2.1.18 support this activity.
GO:0008300 isoprenoid catabolic process
IEA
GO_REF:0000118 		REMOVE
Summary: Isoprenoid catabolism is not supported for KT2440 despite related Liu chemistry in other pseudomonads.
Reason: P. putida KT2440 lacks the acyclic-terpene utilization context and was experimentally unable to grow on acyclic terpenes, while retaining leucine and isovalerate utilization.
Supporting Evidence:
PMID:16820476
P. fluorescens, but not P. putida, could grow on acyclic terpenes
GO:0006552 L-leucine catabolic process
ISS
PMID:16820476
Identification of genes and proteins necessary for catabolis... 		NEW
Summary: LiuC occupies the hydratase position between MCC and HMG-CoA lyase in the distal pathway.
Reason: The specific pathway term is absent from GOA despite the enzyme assignment and conserved ivd-mccB-liuC-mccA cluster.
Supporting Evidence:
PMID:16820476
while both species were able to utilize leucine and isovalerate.
file:PSEPK/liuC/liuC-uniprot.txt
SubName: Full=Methylglutaconyl-CoA hydratase

Core Functions

Hydrates 3-methylglutaconyl-CoA to HMG-CoA in distal L-leucine catabolism.

Molecular Function:
methylglutaconyl-CoA hydratase activity
Directly Involved In:
L-leucine catabolic process
Supporting Evidence:
  • file:PSEPK/liuC/liuC-uniprot.txt
    SubName: Full=Methylglutaconyl-CoA hydratase
  • file:PSEPK/liuC/liuC-deep-research-openscientist.md
    **LiuC (PP_4066) is the soluble cytoplasmic 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18)
  • PMID:16820476
    while both species were able to utilize leucine and isovalerate.

References

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Suggested Experiments

Experiment: Measure hydration of 3-methylglutaconyl-CoA by purified LiuC and test a liuC deletion on leucine, isovalerate, and terpene-derived substrates.

Deep Research

OpenScientist

(liuC-deep-research-openscientist.md)

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📚 Additional Documentation

Notes

(liuC-notes.md)

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