lolA

UniProt ID: Q88FS9
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: COMPLETE
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Gene Description

Periplasmic LolA-family carrier that accepts outer-membrane-destined mature lipoproteins from the inner-membrane LolCDE complex and carries them across the periplasm to LolB for outer-membrane delivery.

Proposed New Ontology Terms

periplasmic lipoprotein carrier activity

Definition: Binding a mature lipid-anchored protein released from the bacterial inner membrane, shielding its lipid moiety during carriage through the periplasm, and presenting it to an outer-membrane acceptor.

Justification: GO:0042953 and GO:0044874 express the process but GO lacks a molecular-function term for the direct carrier activity performed by LolA.

Parent term: molecular_function

Supporting Evidence:

Existing Annotations Review

GO Term Evidence Action Reason
GO:0030288 outer membrane-bounded periplasmic space
IEA
GO_REF:0000118 		ACCEPT
Summary: LolA is a soluble periplasmic lipoprotein carrier in diderm bacteria.
GO:0042597 periplasmic space
IEA
GO_REF:0000120 		MARK AS OVER ANNOTATED
Summary: Correct but redundant with the more taxonomically appropriate outer membrane-bounded periplasmic space annotation.
GO:0042953 lipoprotein transport
IEA
GO_REF:0000120 		ACCEPT
Summary: LolA carries mature lipoproteins through the periplasm.
GO:0044874 lipoprotein localization to outer membrane
IEA
GO_REF:0000120 		ACCEPT
Summary: LolA transfers outer-membrane-destined lipoproteins from LolCDE to LolB.

Core Functions

Periplasmic carriage of mature outer-membrane-destined lipoproteins from the LolCDE release complex to the outer-membrane receptor LolB.

Directly Involved In:
lipoprotein transport lipoprotein localization to outer membrane
Cellular Locations:
outer membrane-bounded periplasmic space
Supporting Evidence:
  • file:PSEPK/lolA/lolA-uniprot.txt
    Participates in the translocation of lipoproteins from the
  • file:modules/bacterial_lol_lipoprotein_trafficking-deep-research-openscientist.md
    LolA is a soluble, monomeric periplasmic chaperone

References

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Suggested Questions for Experts

Q: Does KT2440 LolA apply the canonical Asp-dependent inner-membrane retention rule to all native lipoproteins?

Suggested Experiments

Experiment: Test transfer of purified KT2440 lipoproteins from reconstituted LolCDE to LolA and LolB using +2 residue variants.

Type: lipoprotein transfer assay

📚 Additional Documentation

Notes

(lolA-notes.md)

lolA curation notes

  • Q88FS9 is the reviewed KT2440 LolA ortholog, annotated as a periplasmic outer-membrane lipoprotein carrier [file:PSEPK/lolA/lolA-uniprot.txt, "RecName: Full=Outer-membrane lipoprotein carrier protein"].
  • UniProt places LolA between the inner and outer membranes and records the canonical +2 Asp retention rule [file:PSEPK/lolA/lolA-uniprot.txt, "Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate"].
  • GO:0030288 is retained as the specific diderm-bacterial periplasm location; GO:0042597 is biologically true but redundant.
  • OpenScientist was allowed to run for the full configured 7200 seconds on
    2026-09-01 and returned no report. No provider-named deep-research file was
    synthesized manually; the review remains grounded in the exact target
    UniProt record and the module-level primary/retrieval evidence.

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