lysS

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000049 tRNA binding	IEA GO_REF:0000118	KEEP AS NON CORE	Summary: tRNA binding is inherent but not defining beside lysine-tRNA ligase activity. Reason: The exact reaction identifies tRNA(Lys). Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0000166 nucleotide binding	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: Generic nucleotide binding is uninformative because ATP is specified. Reason: Specific catalytic activity is defining. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0000287 magnesium ion binding	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: Magnesium binding is explicitly rule-assigned but ancillary. Reason: The record predicts three magnesium ions per subunit; no direct KT2440 measurement is available. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Binds 3 Mg(2+) ions per subunit.
GO:0003676 nucleic acid binding	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: Nucleic acid binding is too broad for the specified tRNA substrate. Reason: tRNA(Lys) is explicitly identified. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0004812 aminoacyl-tRNA ligase activity	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: Generic aaRS activity is correct but less informative. Reason: The separately present lysine-specific activity captures the function more precisely. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt RecName: Full=Lysine--tRNA ligase
GO:0004824 lysine-tRNA ligase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is the substrate-specific catalytic activity of LysS. Reason: The exact reaction is assigned by HAMAP rule; homodimerization does not negate the catalytic capability of each polypeptide. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0005524 ATP binding	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: ATP is a substrate but binding is ancillary. Reason: Lysine-tRNA ligase activity is defining. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0005737 cytoplasm	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Cytoplasm is plausible and rule-assigned but not defining. Reason: This is not direct localization evidence. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm
GO:0005829 cytosol	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Cytosol is compatible with cytoplasmic localization but generic and inferred. Reason: Localization is contextual. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm
GO:0006418 tRNA aminoacylation for protein translation	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: The broad process is less informative than lysyl-tRNA aminoacylation. Reason: GO:0006430 already captures the defining substrate-specific process. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0006430 lysyl-tRNA aminoacylation	IEA GO_REF:0000120	ACCEPT	Summary: This is the substrate-specific core process. Reason: The rule-based reaction exactly matches it. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt Reaction=tRNA(Lys) + L-lysine + ATP
GO:0042803 protein homodimerization activity	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Homodimerization is consistent with the rule-assigned LysS quaternary structure but is not the catalytic function. Reason: This ARBA-derived term is supported only by a HAMAP-rule subunit statement. Supporting Evidence: file:PSEPK/lysS/lysS-uniprot.txt SUBUNIT: Homodimer.