mccA

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000166 nucleotide binding	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: Nucleotide binding is implicit in ATP-dependent biotin carboxylation. Reason: Retain this valid but broad binding activity as non-core beside the enzyme-specific biotin carboxylase activity.
GO:0004075 biotin carboxylase activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct alpha-subunit catalytic activity. Reason: MccA contains the biotin-carboxylase and biotin-carrier domains. Supporting Evidence: file:PSEPK/mccA/mccA-uniprot.txt InterPro; IPR011764; Biotin_carboxylation_dom.
GO:0005524 ATP binding	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: ATP binding is mechanistically correct but redundant. Reason: ATP binding is a genuine co-annotatable function of the ATP-grasp biotin-carboxylase domain, but is not the core reaction.
GO:0046872 metal ion binding	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: Generic metal binding does not identify the MCC reaction role. Reason: Retain the mechanistically compatible cofactor-binding annotation as non-core; the specific catalytic activity provides the useful reaction annotation.
GO:0009374 biotin binding	ISM file:PSEPK/mccA/mccA-uniprot.txt	NEW	Summary: MccA contains the biotin-carrier domain and the conserved biotin attachment motif. Reason: Covalently attached biotin is the mobile carboxyl carrier between the alpha-subunit biotin-carboxylase site and the beta-subunit carboxyltransferase site. Supporting Evidence: file:PSEPK/mccA/mccA-uniprot.txt InterPro; IPR000089; Biotin_lipoyl. file:PSEPK/mccA/mccA-uniprot.txt PROSITE; PS00188; BIOTIN; 1.
GO:0004485 methylcrotonoyl-CoA carboxylase activity	ISS PMID:16272386 Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved...	NEW	Summary: MccA supplies biotin carboxylase and carrier functions to the two-subunit MCC holoenzyme. Reason: The complete substrate-specific reaction is a property of assembled MccA-MccB, so contributes_to is more precise than enables for the alpha subunit. Supporting Evidence: PMID:16272386 The two subunits of MCase corresponded to liuB/liuD file:PSEPK/mccA/mccA-uniprot.txt InterPro; IPR048429; MCC_alpha_BT.
GO:0006552 L-leucine catabolic process	ISS PMID:16272386 Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved...	NEW	Summary: The MCC-alpha domain assignment and conserved ivd-mccB-liuC-mccA cluster support a role in distal L-leucine catabolism. Reason: This informative pathway term is absent from the current GOA set. Supporting Evidence: PMID:16272386 The two subunits of MCase corresponded to liuB/liuD file:PSEPK/mccA/mccA-uniprot.txt InterPro; IPR048429; MCC_alpha_BT.
GO:1905202 methylcrotonoyl-CoA carboxylase complex	ISS PMID:16272386 Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved...	NEW	Summary: MccA is the biotin-carboxylase/carrier alpha subunit of bacterial MCC. Reason: Complex membership captures the cooperative MccA-MccB reaction architecture. Supporting Evidence: PMID:16272386 The two subunits of MCase corresponded to liuB/liuD file:PSEPK/mccA/mccA-uniprot.txt InterPro; IPR048429; MCC_alpha_BT.