moaB-II

UniProt ID: Q88E67
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

moaB-II encodes a soluble MoaB-family protein located near canonical moaA. MoaB-family proteins bind GTP and molybdopterin, but catalytic MPT adenylyltransferase activity is lineage-dependent; the exact biochemical contribution and necessity of this KT2440 paralog remain unresolved.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005829 cytosol
IEA
GO_REF:0000118 		KEEP AS NON CORE
Summary: Cytosolic localization is compatible with this small soluble MoaB-family protein.
Reason: Q88E67 lacks membrane/export features and occurs in a soluble cofactor-biosynthesis neighborhood.
GO:0006777 Mo-molybdopterin cofactor biosynthetic process
IEA
GO_REF:0000120 		UNDECIDED
Summary: Pathway involvement is plausible but the paralog's exact contribution and necessity are unresolved.
Reason: UniProt says MoaB may participate but explicitly transfers a no-MPT-adenylyltransferase statement from the family; no gene-specific assay establishes Q88E67's role.
Supporting Evidence:
file:PSEPK/moaB-II/moaB-II-uniprot.txt
CC -!- FUNCTION: May be involved in the biosynthesis of molybdopterin. Can CC bind GTP and has low GTPase activity. Can bind MPT, but has no MPT CC adenylyl transferase activity. {ECO:0000256|ARBA:ARBA00055616}.
file:PSEPK/moaB-II/moaB-II-deep-research-openscientist.md
The caveat is not mis-identification but *evidence type*: there is **no primary experimental study of the P. putida protein Q88E67 itself**; its annotation is by homology to characterized family members.

Core Functions

MoaB-family GTP/MPT-binding protein with an unresolved catalytic or accessory contribution to molybdenum-cofactor biosynthesis.

Supporting Evidence:
  • file:PSEPK/moaB-II/moaB-II-uniprot.txt
    CC -!- FUNCTION: May be involved in the biosynthesis of molybdopterin. Can CC bind GTP and has low GTPase activity. Can bind MPT, but has no MPT CC adenylyl transferase activity. {ECO:0000256|ARBA:ARBA00055616}.
  • file:PSEPK/moaB-II/moaB-II-deep-research-openscientist.md
    - **Family functional ambiguity.** Even in E. coli the role of MoaB is unresolved; this uncertainty propagates to MoaB-II.

References

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Suggested Questions for Experts

Q: Does Q88E67 catalyze MPT adenylylation, act as a noncatalytic pathway partner, or have no required role in KT2440 Mo-cofactor biosynthesis?

Suggested Experiments

Experiment: Compare purified Q88E67 with Q88L15 in MPT-binding, ATP-dependent MPT adenylylation, and MoeA-coupled Mo-MPT formation assays.

Type: comparative biochemical assay

Deep Research

OpenScientist

(moaB-II-deep-research-openscientist.md)

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