mobA

Summary: Generic catalytic activity should be replaced by the exact MobA reaction.

Reason: Reviewed UniProt/HAMAP assigns EC 2.7.7.77 Mo-MPT guanylyltransferase activity.

Proposed replacements: molybdenum cofactor guanylyltransferase activity

Summary: GTP binding is valid substrate binding but less informative than the catalytic term.

Reason: GTP donates GMP in the MobA reaction, and the HAMAP GTP-binding residues are annotated on Q88HA3; the E. coli MobA-GTP cocrystal places GTP in the N-terminal half. Substrate binding is a component of the guanylyltransferase activity, so non-core, as in ECOLI/mobA.

Summary: Cytoplasmic localization is appropriate for this soluble cofactor-maturation enzyme.

Reason: Q88HA3 lacks membrane/export features and acts on soluble Mo-MPT. No cytosol-specific evidence exists for MobA, so cytoplasm is as precise as warranted; same action as ECOLI/mobA.

Summary: MobA participates in the nucleotide-maturation branch of Mo-molybdopterin cofactor biosynthesis.

Reason: Correct but general. The earlier draft accepted the broad term to avoid asserting bis-MGD assembly, but the review already accepts the descendant GO:1902758 (bis-MGD biosynthesis, the TreeGrafter batch-05 verdict), and the E. coli ortholog's role in bis-MGD formation is experimental. The row is refined to GO:1902758, as in ECOLI/mobA.

Proposed replacements: bis(molybdopterin guanine dinucleotide)molybdenum biosynthetic process

Summary: The generic nucleotidyltransferase term should be replaced by the exact MobA activity.

Reason: UniProt specifies GMP transfer from GTP to Mo-MPT.

Proposed replacements: molybdenum cofactor guanylyltransferase activity

Summary: This broad metabolic-process parent is less precise than the retained Mo-molybdopterin cofactor biosynthetic-process annotation.

Reason: Correct but general, so MODIFY rather than the draft's MARK_AS_OVER_ANNOTATED. MobA works in the biosynthetic direction, specifically bis-MGD formation, which the review accepts as GO:1902758; same action as ECOLI/mobA.

Proposed replacements: bis(molybdopterin guanine dinucleotide)molybdenum biosynthetic process

Summary: This is the exact core molecular function of MobA.

Reason: The reviewed entry provides Rhea:34243 and EC 2.7.7.77 for Mo-MPT guanylylation.

Summary: The broad guanylyltransferase term should be replaced by the Mo-cofactor-specific child term.

Reason: The acceptor is specifically Mo-molybdopterin.

Proposed replacements: molybdenum cofactor guanylyltransferase activity

Summary: MobA's guanylyl transfer is the step that builds the bis-MGD cofactor.

Reason: The previous rejection read the single-MGD reaction equation as excluding the bis-MGD product, but bis-MGD is assembled by two successive MobA guanylyl transfers onto Mo-MPT, and the process term is how GO records that contribution: the characterised E. coli ortholog MobA (P32173) carries GO:1902758 with experimental IMP evidence (PMID:1648082) as well as an IBA at PANTHER:PTN001452269 in current GOA. Q88HA3 has the same EC 2.7.7.77 guanylyltransferase assignment and MobA family signature, so the propagated process term describes work this protein performs rather than a downstream event attributed to it.