mupP

id: Q88M11
gene_symbol: mupP
product_type: PROTEIN
status: INITIALIZED
taxon:
  id: NCBITaxon:160488
  label: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
description: mupP encodes the MurNAc-6-phosphate phosphatase that completes the Pseudomonas anabolic peptidoglycan recycling pathway. It converts MurNAc-6P to MurNAc, supports UDP-MurNAc precursor salvage, and contributes to intrinsic fosfomycin resistance.
existing_annotations:
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IEA
  original_reference_id: GO_REF:0000118
  review:
    summary: MupP acts in the cytoplasmic peptidoglycan recycling pathway.
    action: ACCEPT
    reason: Retain cytosolic localization for this recycling enzyme.
    supported_by:
    - reference_id: PMID:28351914
      supporting_text: within the cytoplasm, yielding anhMurNAc
      reference_section_type: INTRODUCTION
- term:
    id: GO:0006281
    label: DNA repair
  evidence_type: IEA
  original_reference_id: GO_REF:0000118
  review:
    summary: DNA repair is not supported for MupP; this appears to be an over-transfer from HAD phosphatase family context.
    action: REMOVE
    reason: MupP is characterized as a MurNAc-6P phosphatase in peptidoglycan recycling, not a DNA repair protein.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: Specifically catalyzes the dephosphorylation of N-
- term:
    id: GO:0008967
    label: phosphoglycolate phosphatase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000118
  review:
    summary: Phosphoglycolate phosphatase activity is not the characterized substrate specificity of MupP.
    action: REMOVE
    reason: The characterized substrate is MurNAc-6P, with narrow substrate specificity.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: Shows a very low activity on GlcNAc-6P
- term:
    id: GO:0016791
    label: phosphatase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: MupP is a phosphatase, but GO lacks a specific MurNAc-6P phosphatase term in these annotations.
    action: ACCEPT
    reason: Retain the broad phosphatase term and propose a more specific ontology term.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: Reaction=N-acetyl-D-muramate 6-phosphate + H2O
- term:
    id: GO:0009254
    label: peptidoglycan turnover
  evidence_type: IEA
  original_reference_id: GO_REF:0000041
  review:
    summary: MupP is directly involved in peptidoglycan recycling/turnover.
    action: ACCEPT
    reason: Retain the peptidoglycan turnover annotation.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: 'PATHWAY: Cell wall biogenesis; peptidoglycan recycling.'
- term:
    id: GO:0009254
    label: peptidoglycan turnover
  evidence_type: IMP
  original_reference_id: PMID:28351914
  review:
    summary: MupP is directly involved in peptidoglycan recycling/turnover.
    action: ACCEPT
    reason: Retain the peptidoglycan turnover annotation.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: 'PATHWAY: Cell wall biogenesis; peptidoglycan recycling.'
- term:
    id: GO:0016791
    label: phosphatase activity
  evidence_type: IDA
  original_reference_id: PMID:28351914
  review:
    summary: MupP is a phosphatase, but GO lacks a specific MurNAc-6P phosphatase term in these annotations.
    action: ACCEPT
    reason: Retain the broad phosphatase term and propose a more specific ontology term.
    supported_by:
    - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
      supporting_text: Reaction=N-acetyl-D-muramate 6-phosphate + H2O
- term:
    id: GO:0097172
    label: N-acetylmuramic acid metabolic process
  evidence_type: IDA
  original_reference_id: PMID:28351914
  review:
    summary: MupP acts on N-acetylmuramate 6-phosphate in the MurNAc recycling pathway.
    action: ACCEPT
    reason: Retain the N-acetylmuramic acid metabolic process annotation.
    supported_by:
    - reference_id: PMID:28351914
      supporting_text: specifically converts MurNAc 6-phosphate to MurNAc
      reference_section_type: ABSTRACT
references:
- id: GO_REF:0000118
  title: TreeGrafter-generated Gene Ontology annotations based on phylogenetic placement.
  findings:
  - statement: Automated source annotation reviewed against UniProt and literature evidence for this gene.
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models.
  findings:
  - statement: Automated source annotation reviewed against UniProt and literature evidence for this gene.
- id: GO_REF:0000041
  title: Gene Ontology annotation based on UniProtKB keyword mapping.
  findings:
  - statement: Automated source annotation reviewed against UniProt and literature evidence for this gene.
- id: PMID:28351914
  title: The N-Acetylmuramic acid 6-phosphate phosphatase MupP completes the Pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance.
  findings:
  - statement: Biochemical and mutant studies identify MupP as the MurNAc-6P phosphatase required for Pseudomonas peptidoglycan recycling and fosfomycin resistance.
- id: file:PSEPK/mupP/mupP-uniprot.txt
  title: UniProtKB reviewed entry for mupP
  findings:
  - statement: UniProt identifies mupP function, pathway placement, catalytic activity, and/or localization used to review GOA annotations.
core_functions:
- description: MupP dephosphorylates MurNAc-6P to MurNAc in the anabolic peptidoglycan recycling route, sustaining UDP-MurNAc precursor salvage and intrinsic fosfomycin resistance.
  molecular_function:
    id: GO:0016791
    label: phosphatase activity
  directly_involved_in:
  - id: GO:0009254
    label: peptidoglycan turnover
  - id: GO:0097172
    label: N-acetylmuramic acid metabolic process
  locations:
  - id: GO:0005829
    label: cytosol
  supported_by:
  - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
    supporting_text: Specifically catalyzes the dephosphorylation of N-
  - reference_id: PMID:28351914
    supporting_text: specifically converts MurNAc 6-phosphate to MurNAc
    reference_section_type: ABSTRACT
  - reference_id: PMID:28351914
    supporting_text: role for MupP in the anabolic PGN recycling route
    reference_section_type: ABSTRACT
proposed_new_terms:
- proposed_name: N-acetylmuramate 6-phosphate phosphatase activity
  proposed_definition: Catalysis of the dephosphorylation of N-acetyl-D-muramate 6-phosphate to N-acetyl-D-muramate and phosphate.
  justification: MupP has a biochemically characterized MurNAc-6P phosphatase activity, but the review is forced to use the overly broad phosphatase activity term.
  proposed_parent:
    id: GO:0016791
    label: phosphatase activity
  supported_by:
  - reference_id: file:PSEPK/mupP/mupP-uniprot.txt
    supporting_text: Reaction=N-acetyl-D-muramate 6-phosphate + H2O
suggested_questions:
- question: How broadly is MupP-dependent anabolic peptidoglycan recycling used across non-enterobacterial Gram-negative lineages lacking MurQ?
  experts:
  - Peptidoglycan recycling and bacterial cell-wall metabolism experts
suggested_experiments:
- description: Compare MurNAc-6P, MurNAc, UDP-MurNAc, and fosfomycin susceptibility in mupP, amgK, and murU mutants across Pseudomonas growth phases.
  experiment_type: cell-wall precursor metabolomics and antibiotic susceptibility assay