mupP

UniProt ID: Q88M11
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

mupP encodes the MurNAc-6-phosphate phosphatase that completes the Pseudomonas anabolic peptidoglycan recycling pathway. It converts MurNAc-6P to MurNAc, supports UDP-MurNAc precursor salvage, and contributes to intrinsic fosfomycin resistance.

Proposed New Ontology Terms

N-acetylmuramate 6-phosphate phosphatase activity

Definition: Catalysis of the dephosphorylation of N-acetyl-D-muramate 6-phosphate to N-acetyl-D-muramate and phosphate.

Justification: MupP has a biochemically characterized MurNAc-6P phosphatase activity, but the review is forced to use the overly broad phosphatase activity term.

Parent term: phosphatase activity

Supporting Evidence:

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005829 cytosol
IEA
GO_REF:0000118 		ACCEPT
Summary: MupP acts on MurNAc-6-phosphate in the cytosolic recycling pathway.
Reason: Full-text fractionation analysis directly tested the PP_1764 deletion mutant and detected recycling intermediates in its cytosolic fraction.
Supporting Evidence:
PMID:28351914
whether recycling intermediates accumulate specifically in the cytosolic fractions of Ξ”mupP (Ξ”pp_1764) mutant cells
GO:0006281 DNA repair
IEA
GO_REF:0000118 		REMOVE
Summary: DNA repair is not supported for MupP; this appears to be an over-transfer from HAD phosphatase family context.
Reason: MupP is characterized as a MurNAc-6P phosphatase in peptidoglycan recycling, not a DNA repair protein.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
Specifically catalyzes the dephosphorylation of N-
GO:0008967 phosphoglycolate phosphatase activity
IEA
GO_REF:0000118 		REMOVE
Summary: Phosphoglycolate phosphatase activity is not the characterized substrate specificity of MupP.
Reason: The characterized substrate is MurNAc-6P, with narrow substrate specificity.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
Shows a very low activity on GlcNAc-6P
GO:0016791 phosphatase activity
IEA
GO_REF:0000117 		ACCEPT
Summary: MupP is a phosphatase, but GO lacks a specific MurNAc-6P phosphatase term in these annotations.
Reason: Retain the broad phosphatase term and propose a more specific ontology term.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
Reaction=N-acetyl-D-muramate 6-phosphate + H2O
GO:0009254 peptidoglycan turnover
IEA
GO_REF:0000041 		ACCEPT
Summary: MupP is directly involved in peptidoglycan recycling/turnover.
Reason: Retain the peptidoglycan turnover annotation.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
PATHWAY: Cell wall biogenesis; peptidoglycan recycling.
GO:0009254 peptidoglycan turnover
IMP
PMID:28351914
The N-Acetylmuramic acid 6-phosphate phosphatase MupP comple... 		ACCEPT
Summary: MupP is directly involved in peptidoglycan recycling/turnover.
Reason: Retain the peptidoglycan turnover annotation.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
PATHWAY: Cell wall biogenesis; peptidoglycan recycling.
GO:0016791 phosphatase activity
IDA
PMID:28351914
The N-Acetylmuramic acid 6-phosphate phosphatase MupP comple... 		ACCEPT
Summary: MupP is a phosphatase, but GO lacks a specific MurNAc-6P phosphatase term in these annotations.
Reason: Retain the broad phosphatase term and propose a more specific ontology term.
Supporting Evidence:
file:PSEPK/mupP/mupP-uniprot.txt
Reaction=N-acetyl-D-muramate 6-phosphate + H2O
GO:0097172 N-acetylmuramic acid metabolic process
IDA
PMID:28351914
The N-Acetylmuramic acid 6-phosphate phosphatase MupP comple... 		ACCEPT
Summary: MupP acts on N-acetylmuramate 6-phosphate in the MurNAc recycling pathway.
Reason: Retain the N-acetylmuramic acid metabolic process annotation.
Supporting Evidence:
PMID:28351914
specifically converts MurNAc 6-phosphate to MurNAc

Core Functions

MupP dephosphorylates MurNAc-6P to MurNAc in the anabolic peptidoglycan recycling route, sustaining UDP-MurNAc precursor salvage and intrinsic fosfomycin resistance.

Molecular Function:
phosphatase activity
Directly Involved In:
peptidoglycan turnover N-acetylmuramic acid metabolic process
Cellular Locations:
cytosol
Supporting Evidence:
  • file:PSEPK/mupP/mupP-uniprot.txt
    Specifically catalyzes the dephosphorylation of N-
  • PMID:28351914
    specifically converts MurNAc 6-phosphate to MurNAc
  • PMID:28351914
    role for MupP in the anabolic PGN recycling route

References

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Suggested Questions for Experts

Q: How broadly is MupP-dependent anabolic peptidoglycan recycling used across non-enterobacterial Gram-negative lineages lacking MurQ?

Suggested experts: Peptidoglycan recycling and bacterial cell-wall metabolism experts

Suggested Experiments

Experiment: Compare MurNAc-6P, MurNAc, UDP-MurNAc, and fosfomycin susceptibility in mupP, amgK, and murU mutants across Pseudomonas growth phases.

Type: cell-wall precursor metabolomics and antibiotic susceptibility assay

πŸ“š Additional Documentation

Notes

(mupP-notes.md)

mupP curation notes

  • PP_1764/Q88M11 was directly identified and biochemically characterized as
    the MurNAc-6-phosphate phosphatase of the KT2440 anabolic recycling route
    [PMID:28351914, "specifically converts MurNAc 6-phosphate to MurNAc"].
  • The TreeGrafter phosphoglycolate-phosphatase and DNA-repair annotations arise
    from the broader HAD phosphatase placement and are contradicted by the direct
    target-specific evidence; both are removed.
  • Cytosol is retained only in the gene review. It is not duplicated as generic
    module-level context.

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