nadE

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity	IEA GO_REF:0000002	REMOVE	Summary: Incorrect nitrogen-donor specificity propagated from a broad family. Reason: The reviewed target is explicitly an NH3-dependent NadE, carries the NH(3)-dependent NAD synthetase subfamily assignment, and lacks a glutaminase domain. Its defined reaction uses NH4+, not glutamine. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt RecName: Full=NH(3)-dependent NAD(+) synthetase file:PSEPK/nadE/nadE-uniprot.txt Reaction=deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) +
GO:0004359 glutaminase activity	IEA GO_REF:0000002	REMOVE	Summary: Incorrect InterPro over-propagation to an ammonia-dependent enzyme. Reason: Q88DF6 is a 275-residue ammonia-dependent NadE with only the NAD synthetase module; no glutaminase catalytic module is present or needed for RHEA:21188. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt InterPro; IPR022926; NH(3)-dep_NAD(+)_synth.
GO:0005524 ATP binding	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: Correct catalytic co-substrate binding, retained as non-core. Reason: ATP is a substrate of the NadE ligase reaction, but GO:0008795 is the informative catalytic function. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt Reaction=deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) +
GO:0005737 cytoplasm	IEA GO_REF:0000120	ACCEPT	Summary: Correct expected location for soluble bacterial NadE. Reason: The compact NadE enzyme is a soluble cytoplasmic protein. The claim rests on family membership plus the absence of export or membrane features; Q88DF6 belongs to the HAMAP MF_00193 ammonia-dependent NadE family (NCBIfam TIGR00552), a soluble homodimeric enzyme family, and the cached record has no SIGNAL, TRANSMEM or lipoprotein feature lines and no CC SUBCELLULAR LOCATION comment that would place it elsewhere. The record's own GO cross-reference is not cited. No KT2440 localization experiment is cached. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt DR HAMAP; MF_00193; NadE_ammonia_dep; 1. file:PSEPK/nadE/nadE-uniprot.txt DR NCBIfam; TIGR00552; nadE; 1.
GO:0008795 NAD+ synthase activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct core molecular function for the ammonia-dependent enzyme. Reason: The reviewed target and HAMAP rule specify the ammonium-dependent deamido-NAD amidation reaction RHEA:21188. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt Reaction=deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) +
GO:0009435 NAD+ biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: Correct core biological process. Reason: NadE performs the final conversion of deamido-NAD to NAD+. Supporting Evidence: file:PSEPK/nadE/nadE-uniprot.txt PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
GO:0016879 ligase activity, forming carbon-nitrogen bonds	IEA GO_REF:0000117	MODIFY	Summary: Correct parent chemistry but too broad for this specific enzyme. Reason: GO:0008795 captures the NAD+ synthase reaction and correct nitrogen-donor class. Proposed replacements: NAD+ synthase activity