opgH encodes a multi-pass inner-membrane GT2/OpgH-family glucosyltransferase that uses UDP-glucose to build the osmoregulated periplasmic glucan backbone with the periplasmic OpgG partner.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Plasma membrane is the appropriate GO cellular-component term for bacterial inner-membrane OpgH. Reason: UniProt places OpgH in the cell inner membrane and records multiple transmembrane helices; GOA maps this bacterial inner-membrane location to plasma membrane. Supporting Evidence: file:PSEPK/opgH/opgH-uniprot.txt SUBCELLULAR LOCATION: Cell inner membrane file:PSEPK/opgH/opgH-uniprot.txt FT TRANSMEM 141..158 file:PSEPK/opgH/opgH-goa.tsv GO:0005886 plasma membrane |
| GO:0009250 glucan biosynthetic process | IEA GO_REF:0000104 | MODIFY | Summary: Glucan biosynthesis is correct but should be made specific to osmoregulated periplasmic glucan biosynthesis. Reason: UniProt and UniPathway specifically place OpgH in osmoregulated periplasmic glucan biosynthesis, and GO provides a specific process term for this pathway. Proposed replacements: osmoregulated periplasmic glucan biosynthetic process Supporting Evidence: file:PSEPK/opgH/opgH-uniprot.txt FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic file:PSEPK/opgH/opgH-uniprot.txt PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG) file:PSEPK/opgH/opgH-goa.tsv GO:0009250 glucan biosynthetic process |
| GO:0016758 hexosyltransferase activity | IEA GO_REF:0000120 | MODIFY | Summary: Hexosyltransferase activity is correct but less informative than glucosyltransferase activity for OpgH. Reason: OpgH is a glucans biosynthesis glucosyltransferase in the glycosyltransferase 2 family, so the child term glucosyltransferase activity better captures the transferred sugar. Proposed replacements: glucosyltransferase activity Supporting Evidence: file:PSEPK/opgH/opgH-uniprot.txt RecName: Full=Glucans biosynthesis glucosyltransferase H file:PSEPK/opgH/opgH-uniprot.txt Belongs to the glycosyltransferase 2 family. OpgH file:PSEPK/opgH/opgH-goa.tsv GO:0016758 hexosyltransferase activity file:PSEPK/opgH/opgH-deep-research-openscientist.md Using cytoplasmic **UDP-glucose** as the sugar donor, OpgH assembles a Ξ²-linked polyglucose chain at the cytoplasmic face of the inner membrane |
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Download this section (compressed HTML)Q: Does KT2440 OpgH use ACP as a cofactor and produce the same beta-1,2/beta-1,6 OPG product structure inferred from characterized orthologs?
Q: How do OpgH and OpgG interact during KT2440 osmoregulated periplasmic glucan production?
Q: Does KT2440 OpgH retain the E. coli-like FtsZ-sequestration moonlighting role, or should that function be explicitly excluded for this organism?
Experiment: Assay opgH deletion or depletion strains for osmoregulated periplasmic glucan production under low-osmolarity growth conditions.
Type: targeted genetics and glucan profiling
Experiment: Reconstitute OpgH membrane fractions with candidate UDP-glucose donor and OpgG to measure glucan polymer formation.
Type: in vitro glycosyltransferase assay
Experiment: Test OpgH-FtsZ colocalization and cell-size effects across nutrient conditions in KT2440.
Type: localization and cell-division phenotype assay
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