id: P0A149
gene_symbol: parA
taxon:
  id: NCBITaxon:160488
  label: Pseudomonas putida KT2440
status: COMPLETE
locus_tag: PP_0002
description: >-
  NCBI PGAP names WP_003253182.1 "ParA family protein" from SPARCLE CDD
  architecture 11439703 (a single COG1192 ParA region) and passes on ATP
  binding and ATP hydrolysis activity. Both are correct. This is a case
  where CDD architecture GO fills a gap that UniProt/GOA leaves completely
  open: GOA has no annotation of any kind for this protein. UniProt keeps the
  Swiss-Prot entry as "Uncharacterized protein PP_0002", and its Pfam
  (AAA_31) and PANTHER (PTHR13696:SF52) matches bring no GO. The main review
  proposes ATP hydrolysis activity as the core function, on the basis of the
  84%-identical P. aeruginosa ParA and the conserved Walker A box, and adds
  ATP binding as a separate row from the same motif. Neither
  pipeline provides the process: chromosome segregation is supported
  directly by P. putida parA mutants, but the ParA architecture carries no
  process term.
references:
- id: PMID:33270901
  title: 'RefSeq: expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation.'
- id: PMID:25139949
  title: Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization
    and interactions with its partner ParB.
- id: file:PSEPK/parA/parA-bioinformatics/RESULTS.md
  title: Pairwise comparison of P. putida and P. aeruginosa ParA and ParB
- id: file:PSEPK/parA/parA-pgap-prediction-evidence/WP_003253182.1.gp
  title: RefSeq GenPept record WP_003253182.1
- id: file:PSEPK/parA/parA-pgap-prediction-evidence/pgap_models.yaml
  title: PGAP naming model and matched NCBI HMMs for parA
source_documents:
- genes/PSEPK/parA/parA-pgap-prediction-evidence/WP_003253182.1.gp
- genes/PSEPK/parA/parA-pgap-prediction-evidence/pgap_models.yaml
predictions:
- source_method: NCBI PGAP
  source_version: GCF_000007565.2-RS_2026_02_04 (live); GO from WP_ record
  source_reference_id: PMID:33270901
  predicted_term:
    id: GO:0005524
    label: ATP binding
  predicted_term_type: GO_MF
  review:
    assessment: COR
    confidence_score: 2
    summary: >-
      Correct and not in GOA. ParA binds ATP through its deviant Walker A box
      (KGGVGKT, residues 11-17), conserved and identically placed in the
      characterized P. aeruginosa ortholog. The main review adds it as a NEW
      row (ISM). GO does not derive ATP binding from ATP hydrolysis activity,
      which sits in a separate branch, so the term carries information of its
      own.
    supported_by:
    - reference_id: file:PSEPK/parA/parA-bioinformatics/RESULTS.md
      supporting_text: ParA has the deviant Walker A box KGGVGKT at 11-17,
        the ATP-binding signature of the ParA/MinD family.
- source_method: NCBI PGAP
  source_version: GCF_000007565.2-RS_2026_02_04 (live); GO from WP_ record
  source_reference_id: PMID:33270901
  predicted_term:
    id: GO:0016887
    label: ATP hydrolysis activity
  predicted_term_type: GO_MF
  review:
    assessment: COR
    confidence_score: 2
    summary: >-
      Correct and not in GOA, which has no annotation for this protein. The
      84%-identical P. aeruginosa ParA hydrolyses ATP as a purified protein.
      The main review proposes this term as the core molecular function (NEW,
      ISO).
    supported_by:
    - reference_id: PMID:25139949
      supporting_text: ParA-His6 demonstrated ATPase activity (Fig. 3) of ~1.2 pmol Pi released min−1
        (pmol ParA)−1.