6-phosphogluconolactonase (6PGL; EC 3.1.1.31) is a cytoplasmic hydrolase that catalyzes the hydrolysis of 6-phospho-D-glucono-1,5-lactone to 6-phospho-D-gluconate. This reaction is the second step of the oxidative branch of glucose-6-phosphate catabolism, following the glucose-6-phosphate dehydrogenase (Zwf) reaction. The 6-phosphogluconate product is the central branch-point metabolite that feeds both the Entner-Doudoroff pathway and the pentose phosphate pathway. In Pseudomonas putida KT2440 the gene (PP_1023) is part of a conserved zwf-pgl-eda operon dedicated to upper glucose catabolism. The protein belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005975 carbohydrate metabolic process | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: General carbohydrate metabolic process term; correct but superseded by the more specific pentose-phosphate shunt annotation. Reason: This is a high-level grouping term. It is not incorrect, but the more specific child term GO:0006098 (pentose-phosphate shunt) is also annotated and better captures the precise biological process for 6-phosphogluconolactonase. Retain as non-core/over-annotated background. |
| GO:0006098 pentose-phosphate shunt | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: 6-phosphogluconolactonase catalyzes step 2 of the oxidative stage of the pentose phosphate pathway (D-ribulose 5-phosphate from D-glucose 6-phosphate), consistent with UniPathway UPA00115 and the enzyme's established role. Reason: Well-supported by enzyme function and pathway membership (UniPathway UER00409, oxidative PPP step 2/3), but GO:0009051 captures the direct oxidative-branch role more precisely. Note that in P. putida the 6-phosphogluconate product predominantly feeds the Entner-Doudoroff pathway. |
| GO:0009051 pentose-phosphate shunt, oxidative branch | ISS file:PSEPK/pgl/pgl-deep-research-openscientist.md | NEW | Summary: Pgl hydrolyzes 6-phosphogluconolactone in the second reaction of the oxidative pentose-phosphate branch. Reason: The oxidative-branch child term is more precise than the existing general pentose-phosphate shunt annotation and matches the enzyme's pathway step. Supporting Evidence: file:PSEPK/pgl/pgl-deep-research-openscientist.md Pgl catalyzes step 2 of the oxidative pentose-phosphate branch |
| GO:0017057 6-phosphogluconolactonase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Catalyzes hydrolysis of 6-phospho-D-glucono-1,5-lactone to 6-phospho-D-gluconate (EC 3.1.1.31; RHEA:12556). This is the precise, defining molecular function of the gene product. Reason: Core molecular function. Supported by UniRule/ARBA annotation, RHEA reaction mapping, EC 3.1.1.31, and family/domain assignment (TIGR01198 pgl; PANTHER PTHR11054; CDD cd01400 6PGL). The protein is a clear member of the 6-phosphogluconolactonase subfamily. Supporting Evidence: file:PSEPK/pgl/pgl-deep-research-openscientist.md Pgl catalyzes step 2 of the oxidative pentose-phosphate branch |
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