pgm (PP_3578) encodes an alpha-D-phosphohexomutase family phosphoglucomutase. It catalyzes the reversible conversion of alpha-D-glucose 1-phosphate and alpha-D-glucose 6-phosphate, linking central hexose-phosphate metabolism with nucleotide-sugar and storage/carbohydrate biosynthetic pools.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000287 magnesium ion binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: magnesium ion binding is biologically plausible for this enzyme but is ancillary to the more specific catalytic function. Reason: Retain as a supporting/non-core annotation rather than using it as the main functional summary. |
| GO:0004614 phosphoglucomutase activity | IEA GO_REF:0000120 | ACCEPT | Summary: phosphoglucomutase activity is consistent with the curated UniProt name, EC/family evidence, and the gene product role summarized here. Reason: This is a specific, biologically appropriate annotation for this gene product. |
| GO:0005975 carbohydrate metabolic process | IEA GO_REF:0000120 | ACCEPT | Summary: carbohydrate metabolic process is consistent with the curated UniProt name, EC/family evidence, and the gene product role summarized here. Reason: This is a specific, biologically appropriate annotation for this gene product. |
| GO:0006166 purine ribonucleoside salvage | IEA GO_REF:0000118 | MARK AS OVER ANNOTATED | Summary: This TreeGrafter process is coupled to a propagated phosphopentomutase assignment, whereas Q88GY7 is specifically classified as an EC 5.4.2.2 phosphoglucomutase in a phosphoglucomutase subfamily. Reason: The broad family contains divergent phosphomutases, but no target-specific evidence connects Q88GY7 to purine ribonucleoside salvage. Supporting Evidence: file:PSEPK/pgm/pgm-uniprot.txt DE EC=5.4.2.2 {ECO:0000256|NCBIfam:TIGR01132}; |
| GO:0008973 phosphopentomutase activity | IEA GO_REF:0000118 | MARK AS OVER ANNOTATED | Summary: This TreeGrafter assignment appears propagated across divergent phosphomutase functions; Q88GY7 is specifically classified as a phosphoglucomutase and lacks target-specific phosphopentomutase evidence. Reason: Retain the established EC 5.4.2.2 activity without importing a distinct pentose-phosphate salvage reaction from deeper family ancestry. Supporting Evidence: file:PSEPK/pgm/pgm-uniprot.txt DR InterPro; IPR005852; PGM_a-D-Glc-sp. |
| GO:0016868 intramolecular phosphotransferase activity | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: intramolecular phosphotransferase activity is biologically plausible for this enzyme but is ancillary to the more specific catalytic function. Reason: Retain as a supporting/non-core annotation rather than using it as the main functional summary. |
| GO:0006011 UDP-alpha-D-glucose metabolic process | ISS file:PSEPK/pgm/pgm-deep-research-openscientist.md | NEW | Summary: Pgm supplies glucose 1-phosphate at the branch point leading to UDP-alpha-D-glucose and downstream nucleotide-sugar-dependent glycans. Supporting Evidence: file:PSEPK/pgm/pgm-deep-research-openscientist.md it functions at the **G1P/G6P branch point** that couples Entner-Doudoroff central carbon metabolism to nucleotide-sugar-dependent biosynthesis (UDP-/ADP-glucose for glycogen, trehalose, LPS/O-antigen, exopolysaccharides, and dTDP-L-rhamnose) |
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Download this section (compressed HTML)Q: How is glucose 1-phosphate flux divided between Pgm and the bifunctional AlgC enzyme under nucleotide-sugar and storage-polymer demand?
Experiment: Measure glucose- and mannose-phosphate mutase kinetics for purified Pgm and quantify pathway flux in pgm and algC perturbation strains.
Type: comparative enzymology and metabolic flux analysis
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