| Claim/Parameter | Value/Observation | Organism/Context | Source (first author year) | URL | Evidence citation id |
|---|---|---|---|---|---|
| Target identity | **ppc / PP_1505 / UniProt Q88MR4** annotated as **phosphoenolpyruvate carboxylase (PEPC/Ppc; EC 4.1.1.31)** | *Pseudomonas putida* KT2440; transcriptomics tables list locus as **PP1505** under pyruvate metabolism | Acuña 2014 | https://doi.org/10.1186/1475-2859-13-88 | (pqac-00000004, pqac-00000005) |
| Reaction stoichiometry | **Phosphoenolpyruvate + HCO3- ⇌ oxaloacetate + Pi**; described as an essentially irreversible bicarbonate-fixation reaction in vivo | Bacterial/plant-type PEPC general biochemistry | Koendjbiharie 2021 | https://doi.org/10.1093/femsre/fuaa061 | (pqac-00000001) |
| Core function | Produces **oxaloacetate (OAA)** from **PEP**, supplying the **PEP-pyruvate-oxaloacetate node** | General bacterial central metabolism | Yin 2024 | https://doi.org/10.3390/molecules29122893 | (pqac-00000003) |
| Biological role | **Anaplerotic enzyme** that replenishes the **TCA cycle** by converting PEP to OAA | Bacteria including *E. coli* and *Corynebacterium glutamicum*; relevant inference for KT2440 Ppc family member | Yin 2024 | https://doi.org/10.3390/molecules29122893 | (pqac-00000000, pqac-00000003) |
| Typical bacterial activators | **Fructose-1,6-bisphosphate** and **acetyl-CoA** activate bacterial PEPC | General bacterial PEPC regulation | Koendjbiharie 2021 | https://doi.org/10.1093/femsre/fuaa061 | (pqac-00000002) |
| Typical bacterial inhibitors | **Aspartate** and **malate** inhibit bacterial PEPC | General bacterial PEPC regulation | Koendjbiharie 2021 | https://doi.org/10.1093/femsre/fuaa061 | (pqac-00000000, pqac-00000002) |
| KT2440 transcriptomic observation | **ppc induction detected** in engineered KT2440 with **pgl** overexpression; discussed as part of altered pyruvate metabolism | *P. putida* KT2440 engineering study on mcl-PHA production | Acuña 2014 | https://doi.org/10.1186/1475-2859-13-88 | (pqac-00000006) |
| KT2440 Table 4 value | **ppc (PP1505)** expression values reported as **0.5** and **0.4**; table note indicates these entries were **not bolded** (not marked as differentiated expression in that table) | *P. putida* KT2440 engineered strains vs parental strains | Acuña 2014 | https://doi.org/10.1186/1475-2859-13-88 | (pqac-00000004) |
| KT2440 Table 5 values | **ppc (PP1505)** fold-change values reported as **0.0** for **Δgcd-pgl** and **1.4** for **KT2440-pgl** | *P. putida* KT2440 engineered strains vs parental strains | Acuña 2014 | https://doi.org/10.1186/1475-2859-13-88 | (pqac-00000005) |
| Localization inference | No organism-specific localization data were retrieved here; as a canonical bacterial PEPC in central carbon metabolism, Ppc is most consistently interpreted as a **cytosolic enzyme** rather than membrane or secreted | Inference from enzyme class and pathway role; direct KT2440 localization evidence not retrieved in cited contexts | Koendjbiharie 2021; Yin 2024 | https://doi.org/10.1093/femsre/fuaa061 ; https://doi.org/10.3390/molecules29122893 | (pqac-00000001, pqac-00000003) |


*Table: This table compiles the key functional-annotation facts for Pseudomonas putida KT2440 ppc/PP_1505, including the PEPC reaction, anaplerotic role, canonical bacterial regulation, and KT2440-specific transcriptomic observations from Acuña et al. 2014.*