proC (PP_3778) encodes pyrroline-5-carboxylate reductase (P5CR; EC 1.5.1.2), the enzyme catalyzing the final step of L-proline biosynthesis. It reduces (S)-1-pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H as the electron donor. This step converts the cyclized intermediate derived from L-glutamate 5-semialdehyde into proline, completing the glutamate-to-proline biosynthetic route. The enzyme is a soluble cytoplasmic protein and provides proline for protein synthesis and for osmotic adaptation in P. putida.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004735 pyrroline-5-carboxylate reductase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Core molecular function. This is the EC 1.5.1.2 activity catalyzing the NAD(P)H-dependent reduction of 1-pyrroline-5-carboxylate to L-proline, matching the UniProt RecName (HAMAP-Rule MF_01925) and the P5C reductase family assignment. Accept as the defining activity of the gene product. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: Correct cellular component. P5C reductase is a soluble cytoplasmic enzyme, consistent with the UniProt SUBCELLULAR LOCATION annotation (Cytoplasm). Accept. |
| GO:0055129 L-proline biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: Core biological process. ProC catalyzes the terminal (step 1/1, P5C to L-proline) reaction of L-proline biosynthesis from L-glutamate 5-semialdehyde, as annotated in the UniProt PATHWAY field. Accept. |
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