pstA

UniProt ID: Q88JJ1
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

PstA membrane permease subunit of a bacterial phosphate ABC transporter. Together with PstC it forms the membrane translocation pathway that receives phosphate from PstS and is energized by PstB. In KT2440, this protein is encoded in the PP_2656-PP_2659 Pst-like operon.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005315 phosphate transmembrane transporter activity
IEA
GO_REF:0000002
MODIFY
Summary: The function is correct at the assembled-permease level, but PstA is one subunit of the PstA/PstC membrane pair and does not independently enable it.
Reason: Retain GO:0005315 with contributes_to rather than enables.
Supporting Evidence:
file:PSEPK/pstA/pstA-uniprot.txt
RecName: Full=Phosphate transport system permease protein PstA
GO:0005886 plasma membrane
IEA
GO_REF:0000120
ACCEPT
Summary: Correct specific membrane location for this multipass permease subunit.
GO:0016020 membrane
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Correct but less precise than the plasma-membrane annotation.
GO:0035435 phosphate ion transmembrane transport
IEA
GO_REF:0000002
ACCEPT
Summary: Directly captures the phosphate-uptake process performed by the Pst complex.
GO:0043190 ATP-binding cassette (ABC) transporter complex
IEA
GO_REF:0000104
MODIFY
Summary: PstA is a membrane component of a substrate-binding-subunit-containing PstSACB transporter.
Reason: Use the specific ABC importer complex term that explicitly includes a substrate-binding subunit.
GO:0055085 transmembrane transport
IEA
GO_REF:0000002
MARK AS OVER ANNOTATED
Summary: Directionally correct but unnecessarily broad relative to phosphate ion transmembrane transport.
Reason: The phosphate-specific process is already present as a separate annotation, making this generic parent redundant.

Core Functions

Contributes the PstA membrane subunit to the PstA/PstC phosphate-selective translocation pathway.

Supporting Evidence:
  • file:PSEPK/pstA/pstA-uniprot.txt
    RecName: Full=Phosphate transport system permease protein PstA

References

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Suggested Questions for Experts

Q: Under which conditions is the PP_2656-PP_2659 Pst-like transporter active, and does PstA assemble exclusively with PstC and PstB1?

Suggested Experiments

Experiment: Reconstitute PstA/PstC with PstB1 or PstB2 and compare phosphate-transport kinetics.

Type: transporter reconstitution

πŸ“š Additional Documentation

Notes

(pstA-notes.md)

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