PstA membrane permease subunit of a bacterial phosphate ABC transporter. Together with PstC it forms the membrane translocation pathway that receives phosphate from PstS and is energized by PstB. In KT2440, this protein is encoded in the PP_2656-PP_2659 Pst-like operon.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005315 phosphate transmembrane transporter activity | IEA GO_REF:0000002 | MODIFY | Summary: The function is correct at the assembled-permease level, but PstA is one subunit of the PstA/PstC membrane pair and does not independently enable it. Reason: Retain GO:0005315 with contributes_to rather than enables. Proposed replacements: phosphate transmembrane transporter activity Supporting Evidence: file:PSEPK/pstA/pstA-uniprot.txt RecName: Full=Phosphate transport system permease protein PstA |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Correct specific membrane location for this multipass permease subunit. |
| GO:0016020 membrane | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Correct but less precise than the plasma-membrane annotation. |
| GO:0035435 phosphate ion transmembrane transport | IEA GO_REF:0000002 | ACCEPT | Summary: Directly captures the phosphate-uptake process performed by the Pst complex. |
| GO:0043190 ATP-binding cassette (ABC) transporter complex | IEA GO_REF:0000104 | MODIFY | Summary: PstA is a membrane component of a substrate-binding-subunit-containing PstSACB transporter. Reason: Use the specific ABC importer complex term that explicitly includes a substrate-binding subunit. Proposed replacements: ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Directionally correct but unnecessarily broad relative to phosphate ion transmembrane transport. Reason: The phosphate-specific process is already present as a separate annotation, making this generic parent redundant. |
Loading supporting contentβ¦
Download this section (compressed HTML)Q: Under which conditions is the PP_2656-PP_2659 Pst-like transporter active, and does PstA assemble exclusively with PstC and PstB1?
Experiment: Reconstitute PstA/PstC with PstB1 or PstB2 and compare phosphate-transport kinetics.
Type: transporter reconstitution
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)