Gene Description

PurE is N5-carboxyaminoimidazole ribonucleotide mutase, which rearranges the PurK product N5-CAIR to CAIR during de novo IMP synthesis.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0003824 catalytic activity	IEA GO_REF:0000117	MODIFY	Summary: Correct but less informative than the enzyme-specific activity. Reason: Replace the generic catalytic parent with N5-CAIR mutase activity. Proposed replacements: 5-(carboxyamino)imidazole ribonucleotide mutase activity
GO:0006189 'de novo' IMP biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: Correct pathway assignment. Reason: This enzyme catalyzes a required reaction between PRPP and IMP.
GO:0034023 5-(carboxyamino)imidazole ribonucleotide mutase activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct enzyme-specific molecular function. Reason: The exact N5-carboxyaminoimidazole ribonucleotide mutase product assignment supports this activity.

Core Functions

Converts N5-CAIR to CAIR in the bacterial two-enzyme carboxylation stage.

Molecular Function:

5-(carboxyamino)imidazole ribonucleotide mutase activity

Directly Involved In:

'de novo' IMP biosynthetic process

Supporting Evidence:

file:PSEPK/purE/purE-uniprot.txt
RecName: Full=N5-carboxyaminoimidazole ribonucleotide mutase
PMID:10574791
catalyzes the unusual rearrangement of N(5)-carboxyaminoimidazole ribonucleotide

References

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Suggested Experiments

Experiment: Test a clean purE deletion for purine auxotrophy and rescue by the appropriate downstream purine intermediate or by gene complementation.

📚 Additional Documentation

Notes

(purE-notes.md)

purE curation notes

UniProt identifies Q88C47 as N5-carboxyaminoimidazole ribonucleotide mutase [file:PSEPK/purE/purE-uniprot.txt "RecName: Full=N5-carboxyaminoimidazole ribonucleotide mutase"].
PurE rearranges N5-CAIR to CAIR in the bacterial two-enzyme route
PMID:10574791.
Replace generic catalytic activity with the exact mutase term GO:0034023.

purE