Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004642 phosphoribosylformylglycinamidine synthase activity	IEA GO_REF:0000120	ACCEPT	Summary: Correct enzyme-specific molecular function. Reason: The exact Phosphoribosylformylglycinamidine synthase product assignment supports this activity.
GO:0005524 ATP binding	IEA GO_REF:0000104	KEEP AS NON CORE	Summary: Valid substrate binding, but not the core function of PurL. Reason: ATP is consumed by the synthase reaction, while the enzyme-specific activity captures the core function.
GO:0005737 cytoplasm	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: Plausible electronic localization that is not core to the enzyme function. Reason: Cytoplasm is consistent with a soluble bacterial metabolic enzyme, but no direct localization evidence was found.
GO:0006164 purine nucleotide biosynthetic process	IEA GO_REF:0000120	MODIFY	Summary: Correct but broader than the specific pathway assignment. Reason: Replace the broad purine-nucleotide process with de novo IMP biosynthesis. Proposed replacements: 'de novo' IMP biosynthetic process
GO:0006189 'de novo' IMP biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: Correct pathway assignment. Reason: This enzyme catalyzes a required reaction between PRPP and IMP.

Core Functions

Converts FGAR to FGAM during de novo IMP synthesis.

Molecular Function:

phosphoribosylformylglycinamidine synthase activity

Directly Involved In:

'de novo' IMP biosynthetic process

Supporting Evidence:

file:PSEPK/purL/purL-uniprot.txt
RecName: Full=Phosphoribosylformylglycinamidine synthase

References

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Suggested Experiments

Experiment: Test a clean purL deletion for purine auxotrophy and rescue by the appropriate downstream purine intermediate or by gene complementation.

📚 Additional Documentation

Notes

(purL-notes.md)

purL curation notes

UniProt identifies Q88P16 as Phosphoribosylformylglycinamidine synthase [file:PSEPK/purL/purL-uniprot.txt "RecName: Full=Phosphoribosylformylglycinamidine synthase"].
Retain ATP binding as non-core because ATP is a reaction substrate. Replace the broad
purine nucleotide process with GO:0006189.

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