PuuB is the predicted gamma-glutamylputrescine oxidase of KT2440. Its exact PANTHER subfamily and close correspondence to experimentally characterized E. coli PuuB support oxidation of gamma-glutamylputrescine to 4-(gamma-glutamylamino)butanal. No dedicated GO molecular-function term currently captures this reaction.
Definition: Catalysis of the oxidation of gamma-L-glutamylputrescine with oxygen and water to form 4-(gamma-L-glutamylamino)butanal, hydrogen peroxide, and ammonium.
Justification: The experimentally characterized Rhea 28414 reaction lacks a substrate-specific GO molecular-function term, forcing PuuB annotations to generic oxidoreductase activity.
Parent term: oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005737 cytoplasm | IEA GO_REF:0000118 | KEEP AS NON CORE | Summary: A cytoplasmic location is plausible but does not describe PuuB's biochemical role. Reason: Retain the generic TreeGrafter location outside the core functional summary. |
| GO:0009447 putrescine catabolic process | ISS UniProtKB:P37906 | NEW | Summary: PuuB performs the second, route-specific oxidation in gamma-glutamyl putrescine catabolism. Reason: The exact PANTHER subfamily and experimentally characterized E. coli exemplar support pathway membership. Supporting Evidence: file:PSEPK/puuB/puuB-uniprot.txt DR PANTHER; PTHR13847:SF275; GAMMA-GLUTAMYLPUTRESCINE OXIDOREDUCTASE; 1. |
| GO:0016641 oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor | ISS UniProtKB:P37906 | NEW | Summary: This is the most specific existing GO parent that captures the oxygen-dependent PuuB reaction class. Reason: Q88K44 shares exact PTHR13847:SF275 membership with characterized E. coli PuuB, and Rhea 28414 oxidizes a CH-NH2 donor using oxygen. A new substrate-specific child term is proposed separately. Supporting Evidence: file:PSEPK/puuB/puuB-uniprot.txt DR PANTHER; PTHR13847:SF275; GAMMA-GLUTAMYLPUTRESCINE OXIDOREDUCTASE; 1. PMID:15590624 oxidization of gamma-glutamylputrescine |
Loading supporting content…
Download this section (compressed HTML)Q: Does KT2440 PuuB have the same substrate range and oxygen dependence as E. coli PuuB?
Experiment: Purify Q88K44 and measure Rhea 28414 turnover with gamma-glutamylputrescine and related glutamylpolyamines.
Type: substrate-specific enzyme kinetics
Q88K44 belongs to PTHR13847:SF275 together with reviewed E. coli PuuB P37906
[file:interpro/panther/PTHR13847/PTHR13847-entries.csv]. The characterized E.
coli pathway explicitly includes "oxidization of gamma-glutamylputrescine"
PMID:15590624. This combination supports the predicted target reaction, while
the absence of a KT2440 assay is retained in the description and experiment
proposal.
The live GO ontology has no substrate-specific term for this reaction. The
verified parent is GO:0016641, oxidoreductase activity acting on a CH-NH2 donor
with oxygen as acceptor. The proposed child is named oxidase activity to match
the oxygen-dependent chemistry and the target UniProt product name.
Loading supporting content…
Download this section (compressed HTML)