puuD

Gene Description

PP_3099 is TssC1, the large sheath subunit of the K1 type VI secretion system. The legacy puuD and urate-oxidase assignments are misleading: TssC-specific domains and the experimentally mapped structural operon identify it as a sheath protein.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004846 urate oxidase activity	IEA GO_REF:0000003	REMOVE	Summary: PP_3099 is a TssC1 sheath protein, not a urate oxidase. Reason: TssC-specific InterPro/PANTHER assignments and the experimentally mapped K1 structural operon contradict the legacy EC mapping. Supporting Evidence: file:PSEPK/puuD/puuD-uniprot.txt T6SS_TssC-like
GO:0033103 protein secretion by the type VI secretion system	ISS PMID:36748579 Transcriptional organization and regulation of the Pseudomon...	NEW	Summary: Forms the contractile K1 sheath together with TssB1; it is not a urate oxidase. Reason: PMID:36748579 and the protein-family/locus evidence place this product in the KT2440 K1-T6SS apparatus. Supporting Evidence: PMID:36748579 The structural operon harbours a total of 15 genes, 12 of which encode the T6SS core components (TssABCDEFGHJKLM) file:PSEPK/puuD/puuD-uniprot.txt TYPE VI SECRETION SYSTEM SHEATH PROTEIN TSSC1

Core Functions

Forms the contractile K1 sheath together with TssB1; it is not a urate oxidase.

Molecular Function:

structural molecule activity

Directly Involved In:

protein secretion by the type VI secretion system

In Complex:

type VI protein secretion system complex

Supporting Evidence:

PMID:36748579
12 of which encode the T6SS core components (TssABCDEFGHJKLM)

References

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Suggested Experiments

Experiment: Compare TssC1 sheath assembly and urate-oxidase activity in wild type, PP_3099 deletion, and complemented strains to test both assignments directly.

Type: sheath imaging and enzyme counter-assay