| Source (first author, year) | Publication type | Organism/strain | What it says about PvdT/PvdRT-OpmQ (function/substrate/localization) | Key quantitative data | URL/DOI |
|---|---|---|---|---|---|
| Stein, 2023 | Dissertation | *Pseudomonas putida* KT2440 | Identifies PvdT as the inner-membrane ABC ATPase component of the tripartite exporter PvdRT-OpmQ. The system is described as a MacB-like/ABC tripartite exporter involved in export and recycling of the siderophore pyoverdine; PvdT works with periplasmic adapter PvdR and outer-membrane channel OpmQ to move substrate to the exterior. Biochemical interaction data support pyoverdine as a ligand/substrate and support PvdT-PvdR complex formation. Deletion phenotypes are consistent with a major but non-exclusive role in pyoverdine secretion. (pqac-00000000, pqac-00000001, pqac-00000002, pqac-00000005) | Deletion of PvdRT-OpmQ reduces pyoverdine secretion by ~50–60%; purification yielded 0.2–0.4 mg PvdR/PvdT from 45 mg total membrane protein. ATPase kinetics reported for PvdT and PvdRT with/without pyoverdine: PvdT Vmax 9.91, KM 0.16 mM, kcat 0.08 s-1, kcat/KM 583.6 M-1 s-1; PvdRT Vmax 69.97, KM 2.58 mM, kcat 0.58 s-1, kcat/KM 214.2 M-1 s-1; PvdT+pyoverdine Vmax 6.43, KM 0.06 mM, kcat 0.05 s-1, kcat/KM 955.9 M-1 s-1; PvdRT+pyoverdine Vmax 40.41, KM 1.30 mM, kcat 0.34 s-1, kcat/KM 241.7 M-1 s-1. (pqac-00000000, pqac-00000001, pqac-00000002) | https://doi.org/10.5282/edoc.32605 |
| Stein, 2023 | Peer-reviewed research article (*Microbiology Spectrum*) | *Pseudomonas putida* KT2440 | States that the ABC-type tripartite efflux system PvdRT-OpmQ mediates secretion of newly synthesized and recycled pyoverdine. Loss of these systems impairs pyoverdine secretion and growth under iron limitation, but effects are partial because of redundancy with other tripartite exporters such as MdtABC-OpmB and ParXY. The excerpt does not resolve subunit-level localization beyond the general tripartite architecture spanning inner membrane, periplasm, and outer membrane. (pqac-00000003, pqac-00000004) | No PvdT-specific kinetic or binding values in the available snippet; qualitative evidence indicates impaired secretion and iron-limited growth upon inactivation of relevant systems, with partial redundancy. (pqac-00000003, pqac-00000004) | https://doi.org/10.1128/spectrum.02300-23 |


*Table: This table summarizes the source-backed evidence available in context for PvdT/PvdRT-OpmQ in *Pseudomonas putida* KT2440. It highlights what each source supports about transporter function, substrate, localization, and any quantitative data useful for functional annotation.*