rbsA-I

Summary: True but less informative than the ATP-binding and ATP-hydrolysis annotations.

Reason: Nucleotide binding is correct but general; the Walker A motifs bind ATP, so the specific child ATP binding (GO:0005524), already on this gene, is preferred.

Proposed replacements: ATP binding

Summary: Correct substrate binding for an ABC energy-coupling ATPase.

Summary: Correct membrane association through the transporter, although RbsA-I is a peripheral ATPase rather than an integral membrane subunit.

Reason: The peripheral ATPase acts on the cytoplasmic face of the inner (plasma) membrane, where the transport activity occurs; accepted, consistent with the E. coli RbsA review.

Summary: Direct catalytic activity that supplies energy to the ABC importer.

Summary: Proposed process annotation for the ATPase subunit of the contiguous RbsABC locus, stated at the monosaccharide level that the family classification supports; D-ribose specificity remains a locus-context inference without independent support for this protein.

Reason: Q88K37 is classified in the InterPro ABC carbohydrate-import ATPase family (IPR050107) and carries both CDD carbohydrate/monosaccharide uptake ABC-ATPase domains (cd03216, cd03215), which places it in the energizing subunits of monosaccharide ABC importers. This proposal was previously D-ribose transmembrane transport (GO:0015752), supported only by the submitter-supplied name (ECO:0000313); D-ribose specificity rests on the neighbouring rbsB/rbsC/rbsK/rbsD genes and on E. coli RbsA orthology, and substrate specificity resides in the binding protein, not the ATPase. The PANTHER subfamily (D-allose import ATPase-related) does not resolve ribose versus allose, so the term is stated at the monosaccharide level. Family-level evidence only; no assay of the KT2440 protein exists.

Summary: Proposed complex membership for the energy-coupling subunit.

Reason: Q88K37 has two PROSITE ABC transporter domains (residues 11-252 and 254-508) and is classified in the InterPro ABC carbohydrate-import ATPase family (IPR050107). Members of this family are the cytoplasmic nucleotide-binding subunits that assemble with a permease and a periplasmic binding protein into an ABC importer, so membership in an ABC transporter complex is inferred from family classification; the complex has not been isolated in KT2440.