RecC is the non-motor recognition and scaffold subunit of the bacterial RecBCD helicase-nuclease complex. It binds DNA within the holoenzyme, stimulates RecB processivity, and helps convert DNA-end unwinding into regulated resection and RecA loading. Pseudomonas RecBCD enzymes do not respond to the Escherichia coli Chi octamer, and the corresponding KT2440 regulatory signal remains unknown.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000724 double-strand break repair via homologous recombination | IEA GO_REF:0000104 | ACCEPT | Summary: RecC is an integral RecBCD subunit required for the end-processing stage of homologous double-strand-break repair. Reason: The target record assigns RecC to RecBCD-mediated recombinational repair, and deletion of recC disables DNA repair in the experimentally studied pseudomonad P. syringae. Supporting Evidence: PMID:20195537 essential for DNA repair and growth of P. syringae at low temperatures file:PSEPK/recC/recC-uniprot.txt facilitates RecA-binding to the ssDNA for homologous DNA recombination |
| GO:0003677 DNA binding | IEA GO_REF:0000104 | ACCEPT | Summary: RecC contacts DNA as part of the RecBCD holoenzyme. Reason: The target UniProt record explicitly states that all three RecBCD subunits contribute to DNA binding. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt All subunits contribute to DNA-binding. |
| GO:0003678 DNA helicase activity | IEA GO_REF:0000104 | REMOVE | Summary: RecC regulates the two helicase motors but is not itself a DNA helicase. Reason: The target record assigns the slow 3-prime-to-5-prime motor to RecB and the fast 5-prime-to-3-prime motor to RecD; RecC instead stimulates RecB processivity and recognizes the regulatory DNA signal. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt RecC stimulates the ATPase and processivity |
| GO:0005524 ATP binding | IEA GO_REF:0000104 | REMOVE | Summary: The imported ATP-binding term is not supported for the non-motor RecC subunit. Reason: This overrides the transferred UniProt ATP-binding keyword: the target record provides no nucleotide-binding site or RecC ATPase reaction and instead says that RecC stimulates the ATPase of RecB. Lack of ATPase catalysis alone would not disprove binding, but the available record supplies no independent positive evidence for ATP binding by RecC. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt RecC stimulates the ATPase and processivity |
| GO:0006310 DNA recombination | IEA GO_REF:0000118 | ACCEPT | Summary: RecC participates in RecBCD-dependent homologous DNA recombination. Reason: P. aeruginosa and P. putida recBCD-like clones restore recombination proficiency, while the target record places RecC in the holoenzyme that prepares and loads recombinogenic DNA ends. Supporting Evidence: PMID:2559208 Three clones from Pseudomonas aeruginosa and Ps. putida conferred recombination proficiency and ATP-dependent nuclease activity file:PSEPK/recC/recC-uniprot.txt facilitates RecA-binding to the ssDNA for homologous DNA recombination |
| GO:0008854 exodeoxyribonuclease V activity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: RecC contributes to Exonuclease V holoenzyme activity but does not independently catalyze DNA cleavage. Reason: The `enables` qualifier incorrectly treats a complex-level activity as an intrinsic RecC molecular function. RecC is the DNA-recognition and processivity subunit; the nuclease active site resides in RecB. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt Heterotrimer of RecB, RecC and RecD. |
| GO:0009338 exodeoxyribonuclease V complex | IEA GO_REF:0000002 | ACCEPT | Summary: RecC is a structural and functional subunit of the Exonuclease V complex. Reason: The target record explicitly identifies the RecB-RecC-RecD heterotrimer, and pseudomonad deletion/complementation experiments require cognate subunits for a functional complex. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt Heterotrimer of RecB, RecC and RecD. PMID:20195537 The RecD requirement is only a function of the RecBCD complex in the bacterium. |
| GO:0140097 catalytic activity, acting on DNA | IEA GO_REF:0000117 | REMOVE | Summary: No independent DNA-catalytic activity is established for RecC. Reason: RecC recognizes DNA and stimulates RecB helicase processivity, whereas RecB and RecD supply motor activity and RecB supplies nuclease catalysis. A generic catalytic-DNA term therefore misrepresents RecC's direct role. Supporting Evidence: file:PSEPK/recC/recC-uniprot.txt RecC stimulates the ATPase and processivity |
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Download this section (compressed HTML)Q: Which DNA sequence or DNA-structure feature is recognized by KT2440 RecC to switch RecBCD from degradative processing toward recombinogenic tail production?
Experiment: Test a clean recC deletion for double-strand-break repair, homologous recombination, and RecA loading, then complement with wild-type protein.
Type: genetic pathway validation
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