Gene Description

rfbA (PP_1783) encodes glucose-1-phosphate thymidylyltransferase in the Pseudomonas putida KT2440 dTDP-L-rhamnose biosynthetic route. It catalyzes formation of dTDP-alpha-D-glucose from dTTP and alpha-D-glucose 1-phosphate, supplying the activated sugar nucleotide for downstream dehydration, epimerization, and reduction to dTDP-L-rhamnose.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0008879 glucose-1-phosphate thymidylyltransferase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is the core molecular function of RfbA in dTDP-L-rhamnose biosynthesis. Reason: UniProt records the EC 2.7.7.24/Rhea reaction forming dTDP-alpha-D-glucose from dTTP and glucose 1-phosphate. Supporting Evidence: file:PSEPK/rfbA/rfbA-uniprot.txt Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.
GO:0019305 dTDP-rhamnose biosynthetic process	ISS file:PSEPK/rfbA/rfbA-uniprot.txt	NEW	Summary: RfbA supplies the dTDP-glucose starting intermediate for dTDP-L-rhamnose biosynthesis. Reason: UniProt explicitly places Q88LZ3 in dTDP-L-rhamnose biosynthesis, even though QuickGO currently only carries the molecular-function annotation. Supporting Evidence: file:PSEPK/rfbA/rfbA-uniprot.txt PATHWAY: Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.

Core Functions

Glucose-1-phosphate thymidylyltransferase that forms dTDP-alpha-D-glucose, the first activated sugar intermediate in dTDP-L-rhamnose biosynthesis.

Molecular Function:

glucose-1-phosphate thymidylyltransferase activity

Directly Involved In:

dTDP-rhamnose biosynthetic process

Supporting Evidence:

file:PSEPK/rfbA/rfbA-uniprot.txt
Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.
file:PSEPK/rfbA/rfbA-uniprot.txt
PATHWAY: Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.

References

Loading supporting content…

Download this section (compressed HTML)

📚 Additional Documentation

Notes

(rfbA-notes.md)

rfbA curation notes

Annotation-reviewer pass (2026-09-01)

Reviewed all 2 selected annotation rows against rfbA-goa.tsv, the local
UniProt record, the four-step module, and the OpenScientist module/pathway/taxon
report. The accepted GO:0008879 activity and proposed GO:0019305 pathway term
match the recorded EC 2.7.7.24 reaction and UniProt pathway statement. Final
actions: 1 ACCEPT, 1 NEW; no PENDING or unresolved existing annotation.

Provenance: [UniProt Q88LZ3, "Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate"] and projects/P_PUTIDA/deep-research/PSEPK__dtdp_l_rhamnose_biosynthesis__ppu00523-deep-research-openscientist.md.

rfbA