Gene Description

rffG (PP_1785) encodes dTDP-glucose 4,6-dehydratase in the Pseudomonas putida KT2440 dTDP-L-rhamnose biosynthetic route. It converts dTDP-alpha-D-glucose to dTDP-4-dehydro-6-deoxy-alpha-D-glucose, producing the deoxy-keto nucleotide-sugar intermediate used by the RmlC/RfbC epimerase step.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0008460 dTDP-glucose 4,6-dehydratase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is the core molecular function of RffG in the dTDP-L-rhamnose pathway. Reason: UniProt records the EC 4.2.1.46/Rhea reaction converting dTDP-alpha-D-glucose to dTDP-4-dehydro-6-deoxy-alpha-D-glucose and water. Supporting Evidence: file:PSEPK/rffG/rffG-uniprot.txt Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose file:PSEPK/rffG/rffG-deep-research-openscientist.md The identity of rffG (PP_1785, Q88LZ1) is unambiguous and fully consistent with the UniProt annotation
GO:0009225 nucleotide-sugar metabolic process	IEA GO_REF:0000002	MODIFY	Summary: Nucleotide-sugar metabolism is correct but broader than the specific dTDP-L-rhamnose pathway role. Reason: The specific process context for this gene cluster is dTDP-rhamnose biosynthesis. Proposed replacements: dTDP-rhamnose biosynthetic process

Core Functions

dTDP-glucose 4,6-dehydratase that converts dTDP-alpha-D-glucose to the deoxy-keto nucleotide sugar used by the downstream dTDP-L-rhamnose epimerase step.

Molecular Function:

dTDP-glucose 4,6-dehydratase activity

Directly Involved In:

dTDP-rhamnose biosynthetic process

Supporting Evidence:

file:PSEPK/rffG/rffG-uniprot.txt
Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose

References

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Deep Research

OpenScientist

(rffG-deep-research-openscientist.md)

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📚 Additional Documentation

Notes

(rffG-notes.md)

rffG curation notes

Annotation-reviewer pass (2026-09-01)

Reviewed all 2 selected annotation rows against rffG-goa.tsv, the local
UniProt record, existing OpenScientist gene research, and the combined module
report. GO:0008460 is the exact EC 4.2.1.46 activity. Replacing broad
GO:0009225 with GO:0019305 records the specific pathway once without a duplicate
NEW assertion. Final actions: 1 ACCEPT, 1 MODIFY; no PENDING or unresolved row.

Provenance: [UniProt Q88LZ1, "Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose"] and projects/P_PUTIDA/deep-research/PSEPK__dtdp_l_rhamnose_biosynthesis__ppu00523-deep-research-openscientist.md.

rffG