sdhC

UniProt ID: Q88FA5
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

Cytochrome b556 membrane anchor subunit of the succinate dehydrogenase/respiratory Complex II enzyme in Pseudomonas putida KT2440. SdhC is a multi-pass inner-membrane protein that, together with SdhD, anchors the SdhAB catalytic head to the plasma membrane and forms the membrane electron-transfer/quinone-interaction domain. It supports the succinate-to-fumarate TCA-cycle step and respiratory quinone reduction by the SdhABCD complex.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005886 plasma membrane
IEA
GO_REF:0000120 		ACCEPT
Summary: Correct localization for the bacterial inner-membrane succinate dehydrogenase cytochrome b subunit.
Reason: UniProt annotates SdhC as a cell inner membrane, multi-pass membrane protein. In Gram-negative bacteria the inner membrane corresponds to the GO plasma membrane.
Supporting Evidence:
file:PSEPK/sdhC/sdhC-uniprot.txt
SUBCELLULAR LOCATION: Cell inner membrane
GO:0006099 tricarboxylic acid cycle
IEA
GO_REF:0000002 		ACCEPT
Summary: Correct TCA-cycle annotation because SdhC is part of the SdhABCD succinate dehydrogenase complex.
Reason: The SdhABCD complex catalyzes the succinate-to-fumarate step of the TCA cycle. SdhC is a membrane anchor/cytochrome b subunit of this required complex.
Supporting Evidence:
file:PSEPK/sdhC/sdhC-uniprot.txt
PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
GO:0009055 electron transfer activity
IEA
GO_REF:0000002 		ACCEPT
Summary: Correct subunit-level role for the cytochrome b membrane domain of succinate dehydrogenase.
Reason: SdhC is the cytochrome b556 membrane subunit of Complex II and contains heme-binding features; it contributes to electron transfer/quinone reduction by the holoenzyme.
Supporting Evidence:
file:PSEPK/sdhC/sdhC-uniprot.txt
Name=heme; Xref=ChEBI:CHEBI:30413;
GO:0016020 membrane
IEA
GO_REF:0000002 		MODIFY
Summary: Correct but less precise than the plasma membrane/inner membrane annotation.
Reason: SdhC is a membrane protein, but UniProt specifies the bacterial cell inner membrane and GOA already has the more informative plasma membrane annotation. Use GO:0005886 as the preferred location term.
Proposed replacements: plasma membrane
Supporting Evidence:
file:PSEPK/sdhC/sdhC-uniprot.txt
SUBCELLULAR LOCATION: Cell inner membrane

Core Functions

Cytochrome b membrane anchor subunit of succinate dehydrogenase/Complex II that contributes electron-transfer and membrane anchoring functions to quinone-linked succinate dehydrogenase activity.

Molecular Function:
electron transfer activity
Directly Involved In:
tricarboxylic acid cycle
Cellular Locations:
plasma membrane
Supporting Evidence:
  • file:PSEPK/sdhC/sdhC-uniprot.txt
    Succinate dehydrogenase cytochrome b556 subunit
  • file:PSEPK/sdhC/sdhC-uniprot.txt
    Name=heme; Xref=ChEBI:CHEBI:30413;

References

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Suggested Questions for Experts

Q: Does SdhC provide the main cytochrome b/heme coordination site in the P. putida KT2440 Complex II membrane domain, and how does it cooperate with SdhD in quinone reduction?

Suggested Experiments

Experiment: Mutate predicted SdhC heme-coordinating residues and assay SdhABCD heme content, quinone binding, membrane assembly, and succinate:quinone activity.

Deep Research

OpenScientist

(sdhC-deep-research-openscientist.md)

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